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In vitro clonal mul...
In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations
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- Safi, Fatemeh (author)
- Lund University,Lunds universitet,Stem Cells and Leukemia,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Dhapola, Parashar (author)
- Lund University,Lunds universitet,Stem Cells and Leukemia,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Erlandsson, Eva (author)
- Lund University,Lunds universitet,Avdelningen för molekylär hematologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Hematology (DMH),Department of Laboratory Medicine,Faculty of Medicine
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- Ulfsson, Linda Geironson (author)
- Lund University,Lunds universitet,Stem Cells and Leukemia,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Calderón, Ariana S. (author)
- Lund University,Lunds universitet,Utvecklingslymfopoes och leukemi,Forskargrupper vid Lunds universitet,Developmental lymphopoiesis and leukemia,Lund University Research Groups
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- Böiers, Charlotta (author)
- Lund University,Lunds universitet,Utvecklingslymfopoes och leukemi,Forskargrupper vid Lunds universitet,Developmental lymphopoiesis and leukemia,Lund University Research Groups
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- Karlsson, Göran (author)
- Lund University,Lunds universitet,Stem Cells and Leukemia,Forskargrupper vid Lunds universitet,Lund University Research Groups
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(creator_code:org_t)
- Elsevier BV, 2023
- 2023
- English.
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In: STAR Protocols. - : Elsevier BV. - 2666-1667. ; 4:1
- Related links:
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http://dx.doi.org/10... (free)
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Abstract
Subject headings
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- Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).1
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Bioinformatics
- Cell Biology
- Cell culture
- Cell isolation
- Flow Cytometry/Mass Cytometry
- Genomics
- Molecular Biology
- Stem Cells
Publication and Content Type
- art (subject category)
- ref (subject category)
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