onr:"swepub:oai:lup.lub.lu.se:085ad1e8-8a03-44a2-8472-fa6fea46966c"
Search: onr:"swepub:oai:lup.lub.lu.se:085ad1e8-8a03-44a2-8472-fa6fea46966c" > Gene delivery to ad...
- 1 of 1
- Previous record
- Next record
- To hitlist
Gene delivery to adult neural stem cells
-
- Falk, A (author)
- Karolinska Institutet
-
- Holmstrom, N (author)
- Karolinska Institutet
-
- Carlen, M (author)
- Karolinska Institutet
- show more...
- Cassidy, R (author)
- show less...
- (creator_code:org_t)
- Elsevier BV, 2002
- 2002
- English.
- In: Experimental Cell Research. - : Elsevier BV. - 1090-2422 .- 0014-4827. ; 279:1, s. 34-39
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Neural stem cells may present an ideal route for gene therapy as well as offer new possibilities for the replacement of neurons lost to injury or disease. However, it has proved difficult to express ectopic genes in stem cells. We report methods to introduce genes into adult neural stem cells using viral and nonviral vectors in vitro and in vivo. Adenoviral and VSV-G-pseudotyped retroviral vectors are more efficient than plasmid transfection or VSV-G lentiviral transduction in vitro. We further show that adult neural stem cells can be directed to a neuronal fate by ectopic expression of neurogenin 2 in vitro. Plasmids can be delivered in vivo when complexed with linear polyethyleneimine, and gene expression can be targeted specifically to neural stem or progenitor cells by the use of specific promoters. These techniques may be utilized both to study the function of various genes in the differentiation of neural stem cells to specific cell fates and, ultimately, for gene therapy or to generate specific differentiated progeny for cell transplantation. (C) 2002 Elsevier Science (USA).
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
- Experimental Cell Research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Falk, A
- Holmstrom, N
- Carlen, M
- Cassidy, R
- Lundberg, Cecili ...
- Frisen, J
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Neurosciences
- Articles in the publication
- Experimental Cel ...
- By the university
- Lund University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
