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- Gustafsson, Mattias C U (author)
Factor H Binds to the Hypervariable Region of Many Streptococcus pyogenes M Proteins but Does Not Promote Phagocytosis Resistance or Acute Virulence.
- Article/chapterEnglish2013
Publisher, publication year, extent ...
- 2013-04-18
- Public Library of Science (PLoS),2013
- electronicrdacarrier
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- LIBRIS-ID:oai:lup.lub.lu.se:08647617-8cad-478e-b5dc-9a8c5cea06cf
- https://lup.lub.lu.se/record/3804988URI
- https://doi.org/10.1371/journal.ppat.1003323DOI
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- Language:English
- Summary in:English
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Notes
- Many pathogens express a surface protein that binds the human complement regulator factor H (FH), as first described for Streptococcus pyogenes and the antiphagocytic M6 protein. It is commonly assumed that FH recruited to an M protein enhances virulence by protecting the bacteria against complement deposition and phagocytosis, but the role of FH-binding in S. pyogenes pathogenesis has remained unclear and controversial. Here, we studied seven purified M proteins for ability to bind FH and found that FH binds to the M5, M6 and M18 proteins but not the M1, M3, M4 and M22 proteins. Extensive immunochemical analysis indicated that FH binds solely to the hypervariable region (HVR) of an M protein, suggesting that selection has favored the ability of certain HVRs to bind FH. These FH-binding HVRs could be studied as isolated polypeptides that retain ability to bind FH, implying that an FH-binding HVR represents a distinct ligand-binding domain. The isolated HVRs specifically interacted with FH among all human serum proteins, interacted with the same region in FH and showed species specificity, but exhibited little or no antigenic cross-reactivity. Although these findings suggested that FH recruited to an M protein promotes virulence, studies in transgenic mice did not demonstrate a role for bound FH during acute infection. Moreover, phagocytosis tests indicated that ability to bind FH is neither sufficient nor necessary for S. pyogenes to resist killing in whole human blood. While these data shed new light on the HVR of M proteins, they suggest that FH-binding may affect S. pyogenes virulence by mechanisms not assessed in currently used model systems.
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- Lannergård, JonasLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)med-jlg (author)
- Nilsson, Rickard OLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)med-ran (author)
- Kristensen, BodilLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)med-bkr (author)
- Olsen, John E (author)
- Harris, Claire L (author)
- Ufret-Vincenty, Rafael L (author)
- Stålhammar-Carlemalm, MargarethaLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-msc (author)
- Lindahl, GunnarLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-gli (author)
- Avdelningen för medicinsk mikrobiologiInstitutionen för laboratoriemedicin (creator_code:org_t)
Related titles
- In:PLoS Pathogens: Public Library of Science (PLoS)9:41553-73661553-7374
Internet link
- https://portal.research.lu.se/files/2062831/4092252.pdfFULLTEXT
- http://www.ncbi.nlm.nih.gov/pubmed/23637608?dopt=AbstractFULLTEXT
- http://dx.doi.org/10.1371/journal.ppat.1003323FULLTEXT
- https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1003323&type=printable
- https://lup.lub.lu.se/record/3804988
- https://doi.org/10.1371/journal.ppat.1003323
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