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Linoleic acid-stimu...
Linoleic acid-stimulated vascular adhesion molecule-1 expression in endothelial cells depends on nuclear factor-kappaB activation.
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Dichtl, Wolfgang (author)
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- Ares, Mikko (author)
- Lund University,Lunds universitet,Kardiovaskulär forskning - immunitet och ateroskleros,Forskargrupper vid Lunds universitet,Cardiovascular Research - Immunity and Atherosclerosis,Lund University Research Groups
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Niemann-Jönson, Audrey (author)
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- Jovinge, Stefan (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine
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Pachinger, Otmar (author)
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Giachelli, Cecilia M (author)
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- Hamsten, Anders (author)
- Karolinska Institutet
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- Eriksson, Per (author)
- Karolinska Institutet
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- Nilsson, Jan (author)
- Lund University,Lunds universitet,Kardiovaskulär forskning - immunitet och ateroskleros,Forskargrupper vid Lunds universitet,Cardiovascular Research - Immunity and Atherosclerosis,Lund University Research Groups
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(creator_code:org_t)
- Elsevier BV, 2002
- 2002
- English.
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In: Metabolism, Clinical and Experimental. - : Elsevier BV. - 1532-8600 .- 0026-0495. ; 51:3, s. 327-333
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Abstract
Subject headings
Close
- Endothelial activation is an important step in atherogenesis. In addition to established cardiovascular risk factors, such as hypercholesterolemia, hypertension, diabetes mellitus, and homocysteinemia, high plasma levels of triglyceride-rich lipoproteins may be an important cause of endothelial activation as well. Free fatty acids hydrolyzed from core triglycerides of these particles can exert both pro- and anti-inflammatory effects on the vascular wall. omega-3 fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have been shown to inhibit cytokine-induced endothelial activation. In contrast, we and others have previously shown that the omega-6 fatty acid linoleate activates transcription factor nuclear factor-kappaB (NF-kappaB) in endothelial cells. In this study, we show that linoleic acid stimulates vascular adhesion molecule-1 (VCAM-1) protein and mRNA expression in cultured human endothelial cells, as assessed by immunofluorescence and Northern blotting. Release of shedded soluble VCAM-1 from cultured human endothelial cells was also increased by the addition of linoleic acid, as determined by enzyme-linked immunosorbent assay (ELISA). By use of cultured rat aortic endothelial cells transfected with an IkappaB super-repressor (DeltaN2 cells), we provide evidence that NF-kappaB signalling is required in the linoleic acid-induced VCAM-1 expression in endothelial cells, whereas other transcription factors appear to be involved in the increased endothelial plasminogen activator inhibitor-1 (PAI-1) production in response to linoleic acid. These findings suggest that diets rich in linoleic acid may be proinflammatory and thus atherogenic by activating vascular endothelial cells.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Reverse Transcriptase Polymerase Chain Reaction
- Support
- Non-U.S. Gov't
- U.S. Gov't
- P.H.S.
- Vascular Cell Adhesion Molecule-1/genetics/*metabolism
- Linoleic Acid/*pharmacology
- NF-kappa B/*physiology
- Plasminogen Activator Inhibitor 1/metabolism
- Human
- Fluorescent Antibody Technique
- Vascular/cytology/*metabolism
- Enzyme-Linked Immunosorbent Assay
- RNA
- Messenger/metabolism
- Endothelium
- Cultured
- Cells
- Northern
- Blotting
- Aorta/cytology/metabolism
- Animal
- Rats
Publication and Content Type
- art (subject category)
- ref (subject category)
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To the university's database
- By the author/editor
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Dichtl, Wolfgang
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Ares, Mikko
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Niemann-Jönson, ...
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Jovinge, Stefan
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Pachinger, Otmar
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Giachelli, Cecil ...
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show more...
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Hamsten, Anders
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Eriksson, Per
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Nilsson, Jan
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Metabolism, Clin ...
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Metabolism
- By the university
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Lund University
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Karolinska Institutet