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The pentatricopepti...
The pentatricopeptide repeat protein EMP603 is required for the splicing of mitochondrial Nad1 intron 2 and seed development in maize
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- Fan, Kaijian (author)
- Chinese Academy of Agricultural Sciences
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- Ren, Zhenjing (author)
- Chinese Academy of Agricultural Sciences
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- Zhang, Xiaofeng (author)
- Chinese Academy of Agricultural Sciences
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- Liu, Yan (author)
- Chinese Academy of Agricultural Sciences
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- Fu, Junjie (author)
- Chinese Academy of Agricultural Sciences
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- Qi, Chunlai (author)
- Chinese Academy of Agricultural Sciences
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- Tatar, Wurinile (author)
- Chinese Academy of Agricultural Sciences
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- Rasmusson, Allan G. (author)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Växtbiologi,Forskargrupper vid Lunds universitet,Molecular Cell Biology,Department of Biology,Faculty of Science,Plant Biology,Lund University Research Groups
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- Wang, Guoying (author)
- Chinese Academy of Agricultural Sciences
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- Liu, Yunjun (author)
- Chinese Academy of Agricultural Sciences
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(creator_code:org_t)
- 2021-07-19
- 2021
- English 16 s.
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In: Journal of Experimental Botany. - : Oxford University Press (OUP). - 0022-0957 .- 1460-2431. ; 72:20, s. 6933-6948
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http://dx.doi.org/10...
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Abstract
Subject headings
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- Intron splicing is an essential event in post-transcriptional RNA processing in plant mitochondria, which requires the participation of diverse nuclear-encoded splicing factors. However, it is presently unclear how these proteins cooperatively take part in the splicing of specific introns. In this study, we characterized a nuclear-encoded mitochondrial P-type pentatricopeptide repeat (PPR) protein named EMP603. This protein is essential for splicing of intron 2 in the Nad1 gene and interacts with the mitochondria-localized DEAD-box RNA helicase PMH2-5140, the RAD52-like proteins ODB1-0814 and ODB1-5061, and the CRM domain-containing protein Zm-mCSF1. Further study revealed that the N-terminal region of EMP603 interacts with the DEAD-box of PMH2-5140, the CRM domain of Zm-mCSF1, and OBD1-5061, but not with OBD1-0814, whereas the PPR domain of EMP603 can interact with ODB1-0814, ODB1-5061, and PMH2-5140, but not with Zm-mCSF1. Defects in EMP603 severely disrupt the assembly and activity of mitochondrial complex I, leading to impaired mitochondrial function, and delayed seed development. The interactions revealed between EMP603 and PMH2-5140, ODB1-0814, ODB1-5061, and Zm-mCSF1 indicate a possible involvement of a dynamic 'spliceosome-like' complex in intron splicing, and may accelerate the elucidation of the intron splicing mechanism in plant mitochondria.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Emp603
- intron splicing
- mitochondrion
- PPR protein
- seed development
- Zea mays
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Fan, Kaijian
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Ren, Zhenjing
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Zhang, Xiaofeng
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Liu, Yan
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Fu, Junjie
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Qi, Chunlai
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show more...
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Tatar, Wurinile
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Rasmusson, Allan ...
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Wang, Guoying
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Liu, Yunjun
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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Journal of Exper ...
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Lund University