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Rapid microwave-sti...
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Ruijter, Emiel ThRadboud University Medical Center
(author)
Rapid microwave-stimulated fixation of entire prostatectomy specimens
- Article/chapterEnglish1997
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LIBRIS-ID:oai:lup.lub.lu.se:0e80b0b2-af49-4a08-b826-ca77bbd8819a
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https://lup.lub.lu.se/record/0e80b0b2-af49-4a08-b826-ca77bbd8819aURI
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https://doi.org/10.1002/(SICI)1096-9896(199711)183:3<369::AID-PATH929>3.0.CO;2-9DOI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Conventional fixation of large solid surgical specimens is a slow process. Consequently, autolytic damage to tissues may occur if the fixative does not reach the central part of the specimen in time. However, as there is also a time relationship between formalin fixation and antigen masking, fixation for too long can also be detrimental. In seeking the optimum balance for fixation, microwave irradiation might be of assistance. This study set out to evaluate methods for fixing entire prostate glands within a brief period of time, using microwave-stimulated formalin fixation. The results show that entire prostates can be optimally fixed if formalin is present throughout the tissue as the temperature is increased by microwave irradiation. This is achieved by injecting the fixative into the prostate at multiple sites immediately following prostatectomy. The technique described ensures standardization of a critical step during tissue processing, leading to uniform microscopic results with both routine and immunohistochemical stains. It is a simple, rapid method, suitable for routine diagnostic use. Using this modified approach, DNA of much larger sizes can be extracted from paraffin-embedded material, which could expand the possibilities for molecular analysis.
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Miller, Gary J.University of Colorado
(author)
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Aalders, Tilly W.Radboud University Medical Center
(author)
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Van De Kaa, Christina A.Radboud University Medical Center
(author)
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Schalken, Jack A.Radboud University Medical Center
(author)
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Debruyne, Frans M.Radboud University Medical Center
(author)
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Boon, Mathilde E.Leiden Cytology and Pathology Laboratory
(author)
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Schröder, F. H.
(creator_code:cre_t)
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Lilja, H.Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-hli
(creator_code:cre_t)
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Robson, C. N.
(creator_code:cre_t)
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Radboud University Medical CenterUniversity of Colorado
(creator_code:org_t)
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In:Journal of Pathology183:3, s. 369-3750022-3417
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Ruijter, Emiel T ...
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Miller, Gary J.
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Aalders, Tilly W ...
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Van De Kaa, Chri ...
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Schalken, Jack A ...
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Debruyne, Frans ...
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Boon, Mathilde E ...
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Schröder, F. H.
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Lilja, H.
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Robson, C. N.
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- MEDICAL AND HEALTH SCIENCES
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Lund University