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Comparing different techniques for obtaining molecular size distributions of glycogen

Wan, Yujun (author)
University of Queensland
Chua, Sheena M.H. (author)
University of Queensland
Yao, Yin (author)
University of New South Wales
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Adler, Lewis (author)
University of New South Wales
Navarro, Marta (author)
University of Queensland
Roura, Eugeni (author)
University of Queensland
Tilley, Richard D. (author)
University of New South Wales
Li, Changfeng (author)
Yangzhou University
Nilsson, Lars (author)
Lund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
Gilbert, Robert G. (author)
Yangzhou University,University of Queensland
Sullivan, Mitchell A. (author)
University of Queensland
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 (creator_code:org_t)
2023
2023
English.
In: European Polymer Journal. - 0014-3057. ; 201
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Glycogen is a hyperbranched glucose polymer, serving as a major energy reservoir in animals. Characterization of the size distributions of complex branched polysaccharides helps in the understanding of their structural and functional properties. This study compared the effectiveness of various techniques in analyzing glycogen size distributions, namely size-separation-based techniques (size exclusion chromatography (SEC) and asymmetric-flow field-flow fractionation (AF4)) and imaging-based methods (transmission electron microscopy (TEM) and atomic force microscopy (AFM)). SEC and AF4 gave similar glycogen size distributions. TEM was suboptimal for obtaining size distributions due to the difficulty of preventing aggregation artifacts during sample preparation. AFM appeared to avoid the problem, but the size distributions so obtained had substantially lower resolution and were much more laborious to acquire. The microscopy-based techniques, especially TEM, do however provide richer morphological information on individual particles than do SEC and AF4. These results suggest that combining size-separation and microscopy (imaging) techniques could provide a holistic view of glycogen size distribution and morphology, which may also be applicable to the characterization of other complex branched polymers.

Subject headings

NATURVETENSKAP  -- Kemi -- Polymerkemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Keyword

Asymmetric-flow field-flow fractionation
Atomic force microscopy
Glycogen
Size distribution
Size exclusion chromatography
Transmission electron microscopy

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art (subject category)
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