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Increased insulin c...
Increased insulin clearance in mice with double deletion of glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide receptors
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- Tura, Andrea (author)
- CNR Institute of Neuroscience, Pisa
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- Bizzotto, Roberto (author)
- CNR Institute of Neuroscience, Pisa
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- Yamada, Yuchiro (author)
- Akita University,CNR Institute of Neuroscience, Pisa
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- Seino, Yutaka (author)
- CNR Institute of Neuroscience, Pisa,Kansai Electric Power Hospital
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- Pacini, Giovanni (author)
- CNR Institute of Neuroscience, Pisa
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- Ahrén, Bo (author)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Department of Clinical Sciences, Lund,Faculty of Medicine,CNR Institute of Neuroscience, Pisa
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(creator_code:org_t)
- American Physiological Society, 2018
- 2018
- English.
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In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. - : American Physiological Society. - 0363-6119 .- 1522-1490. ; 314:5, s. 639-646
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http://dx.doi.org/10...
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Abstract
Subject headings
Close
- To establish whether incretin hormones affect insulin clearance, the aim of this study was to assess insulin clearance in mice with genetic deletion of receptors for both glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), so called double incretin receptor knockout mice (DIRKO). DIRKO (n = 31) and wild-type (WT) C57BL6J mice (n = 45) were intravenously injected with D-glucose (0.35 g/kg). Blood was sampled for 50 min and assayed for glucose, insulin, and C-peptide. Data were modeled to calculate insulin clearance; C-peptide kinetics was established after human C-peptide injection. Assessment of C-peptide kinetics revealed that C-peptide clearance was 1.66 ± 0.10 10–31/min. After intravenous glucose administration, insulin clearance during first phase insulin secretion was markedly higher in DIRKO than in WT mice (0.68 ± 0.06 10–3l/min in DIRKO mice vs. 0.54 ± 0.03 10–31/min in WT mice, P = 0.02). In contrast, there was no difference between the two groups in insulin clearance during second phase insulin secretion (P = 0.18). In conclusion, this study evaluated C-peptide kinetics in the mouse and exploited a mathematical model to estimate insulin clearance. Results showed that DIRKO mice have higher insulin clearance than WT mice, following intravenous injection of glucose. This suggests that incretin hormones reduce insulin clearance at physiological, nonstimulated levels.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- Animal model
- Incretin hormones
- Insulin clearance
- Insulin secretion
- Mathematical model
Publication and Content Type
- art (subject category)
- ref (subject category)
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