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Organic solvent cha...
Organic solvent changes the chymotrypsin specificity with respect to nucleophiles
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- Gololobov, Mikhail Yu (author)
- Lund University
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- Voyushina, Tatyana L. (author)
- State Research Institute of Genetics and Selection of Industrial Microorganisms of NRC «Kurchatov Institute»
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- Stepanov, Valentin M. (author)
- State Research Institute of Genetics and Selection of Industrial Microorganisms of NRC «Kurchatov Institute»
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- Adlercreutz, Patrick (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- 1992
- 1992
- English 4 s.
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In: FEBS Letters. - 0014-5793. ; 307:3, s. 309-312
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http://dx.doi.org/10... (free)
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Abstract
Subject headings
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- In α-chymotrypsin-catalyzed acyl-transfer reactions in water the specificity of the enzyme (the nucleophile reactivity of amino acid amides) is correlated with the substrate hydrophobicity and increases as the hydrophobicity of the side chain of the amino acid amides is increased. In a low water system (4% H2O) bulky amino acid amides are less efficient nucleophiles. The specificity of α-chymotrypsin towards the amino acid amides in acyl transfer reactions in this case does not depend on the hydrophobicity of the amino acid side chains but correlates with their size. Therefore, different factors can be responsible for the specificity of enzymes in water and in a mainly organic medium.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Keyword
- Low water system
- Nucleophile specificity
- QSAR
- α-Chymotrypsin
Publication and Content Type
- art (subject category)
- ref (subject category)
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