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Optical projection ...
Optical projection tomography for rapid whole mouse brain imaging
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- Nguyen, David (author)
- Swiss Federal Institute of Technology
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- Marchand, Paul J. (author)
- Swiss Federal Institute of Technology
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- Planchette, Arielle L. (author)
- Swiss Federal Institute of Technology
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- Nilsson, Julia (author)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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- Sison, Miguel (author)
- Swiss Federal Institute of Technology
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- Extermann, Jérôme (author)
- Swiss Federal Institute of Technology
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- Lopez, Antonio (author)
- Swiss Federal Institute of Technology
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- Sylwestrzak, Marcin (author)
- Swiss Federal Institute of Technology
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- Sordet-Dessimoz, Jessica (author)
- Swiss Federal Institute of Technology
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- Schmidt-Christensen, Anja (author)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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- Holmberg, Dan (author)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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- Van De Ville, Dimitri (author)
- Swiss Federal Institute of Technology
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- Lasser, Theo (author)
- Swiss Federal Institute of Technology
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(creator_code:org_t)
- 2017
- 2017
- English 14 s.
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In: Biomedical Optics Express. - 2156-7085. ; 8:12, s. 5637-5650
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http://dx.doi.org/10... (free)
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Abstract
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- In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
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- art (subject category)
- ref (subject category)
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- By the author/editor
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Nguyen, David
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Marchand, Paul J ...
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Planchette, Arie ...
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Nilsson, Julia
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Sison, Miguel
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Extermann, Jérôm ...
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show more...
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Lopez, Antonio
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Sylwestrzak, Mar ...
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Sordet-Dessimoz, ...
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Schmidt-Christen ...
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Holmberg, Dan
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Van De Ville, Di ...
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Lasser, Theo
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Radiology Nuclea ...
- Articles in the publication
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Biomedical Optic ...
- By the university
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Lund University