onr:"swepub:oai:lup.lub.lu.se:12327294-ec91-45b7-a43d-ca9d600b5b7e"
Search: onr:"swepub:oai:lup.lub.lu.se:12327294-ec91-45b7-a43d-ca9d600b5b7e" > Movement of the Int...
- 1 of 1
- Previous record
- Next record
- To hitlist
Movement of the Intermediate and Rate Determining Transition State of Barnase on the Energy Landscape with Changing Temperature
- Dalby, Paul A (author)
-
- Oliveberg, Mikael (author)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
- Fersht, Alan R (author)
- (creator_code:org_t)
- 1998-03-13
- 1998
- English.
- In: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 37:13, s. 4674-4679
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Barnase folds cooperatively via an intermediate, followed by a rate-limiting transition state. We have probed possible movements of the intermediate and transition state on the energy landscape with changing temperature, from the temperature dependence of -values. These measure interaction energies at the level of individual residues. The results suggest that single destabilizing mutations can redistribute the structures in each ensemble on the energy landscape as the temperature is varied. The results were also analyzed in terms of the bulk properties of each ensemble and their movements on the energy landscape. These movements can be described in terms of the "new view" or equivalently in terms of the classical "Hammond" or "anti-Hammond" effects, observed previously for the transition states of barnase at 7.25 M urea and chymotrypsin inhibitor 2 (CI2) at 0.3 and 6 M GdmCl. The results presented here are under more relevant physiological conditions, free of chemical denaturants. The "average" structures of the intermediate and the transition state do not appear to move on the energy landscape as the temperature is varied. However, there are small rearrangements in the major -helix of the transition state, its average structure moving closer to the native state as the temperature is increased, in agreement with the Hammond effect observed previously.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Dalby, Paul A
- Oliveberg, Mikae ...
- Fersht, Alan R
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Biochemistry and ...
- Articles in the publication
- Biochemistry
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
