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Multicenter prospec...
Multicenter prospective evaluation of diagnostic potential of flow cytometric aberrancies in myelodysplastic syndromes by the ELN iMDS flow working group
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- Kern, Wolfgang (author)
- Munich Leukemia Laboratory (MLL)
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- Westers, Theresia M. (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
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- Bellos, Frauke (author)
- Munich Leukemia Laboratory (MLL)
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- Bene, Marie Christine (author)
- Nantes University Hospital
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- Bettelheim, Peter (author)
- Elisabethinen Hospital Linz
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- Brodersen, Lisa Eidenschink (author)
- Hematologics Inc.
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- Burbury, Kate (author)
- Peter MacCallum Cancer Centre
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Chu, Sung Chao (author)
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- Cullen, Matthew (author)
- St James's University Hospital
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- Porta, Matteo Della (author)
- Humanitas Research Hospital
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- Dunlop, Alan Stewart (author)
- UCL Cancer Institute
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- Johansson, Ulrika (author)
- University Hospitals Bristol NHS Foundation Trust
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- Matarraz, Sergio (author)
- University of Salamanca
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- Oelschlaegel, Uta (author)
- Dresden University of Technology
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Ogata, Kiyoyuki (author)
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- Porwit, Anna (author)
- Lund University,Lunds universitet,Patologi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Pathology, Lund,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Preijers, Frank (author)
- Radboud University Medical Center
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- Psarra, Katherina (author)
- Evangelismos Athens General Hospital
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- Saft, Leonie (author)
- Karolinska Institutet,Karolinska University Hospital
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- Subirá, Dolores (author)
- Hospital Universitario de Guadalajara
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- Weiß, Elisabeth (author)
- Munich Leukemia Laboratory (MLL)
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- van der Velden, Vincent H.J. (author)
- Erasmus University Medical Center
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- van de Loosdrecht, Arjan (author)
- Amsterdam UMC - Vrije Universiteit Amsterdam
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Weiss, E (author)
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(creator_code:org_t)
- 2022-11-23
- 2023
- English 15 s.
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In: Cytometry Part B - Clinical Cytometry. - : Wiley. - 1552-4949 .- 1552-4957. ; 104:1, s. 51-65
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Abstract
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- Background: Myelodysplastic syndromes (MDS) represent a diagnostic challenge. This prospective multicenter study was conducted to evaluate pre-defined flow cytometric markers in the diagnostic work-up of MDS and chronic myelomonocytic leukemia (CMML). Methods: Thousand six hundred and eighty-two patients with suspected MDS/CMML were analyzed by both cytomorphology according to WHO 2016 criteria and flow cytometry according to ELN recommendations. Flow cytometric readout was categorized ‘non-MDS’ (i.e. no signs of MDS/CMML and limited signs of MDS/CMML) and ‘in agreement with MDS’ (i.e., in agreement with MDS/CMML). Results: Flow cytometric readout categorized 60% of patients in agreement with MDS, 28% showed limited signs of MDS and 12% had no signs of MDS. In 81% of cases flow cytometric readouts and cytomorphologic diagnosis correlated. For high-risk MDS, the level of concordance was 92%. A total of 17 immunophenotypic aberrancies were found independently related to MDS/CMML in ≥1 of the subgroups of low-risk MDS, high-risk MDS, CMML. A cut-off of ≥3 of these aberrancies resulted in 80% agreement with cytomorphology (20% cases concordantly negative, 60% positive). Moreover, >3% myeloid progenitor cells were significantly associated with MDS (286/293 such cases, 98%). Conclusion: Data from this prospective multicenter study led to recognition of 17 immunophenotypic markers allowing to identify cases ‘in agreement with MDS’. Moreover, data emphasizes the clinical utility of immunophenotyping in MDS diagnostics, given the high concordance between cytomorphology and the flow cytometric readout. Results from the current study challenge the application of the cytomorphologically defined cut-off of 5% blasts for flow cytometry and rather suggest a 3% cut-off for the latter.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Keyword
- flow cytometry
- hematology
- multiparameter analysis
- myelodysplastic syndrome
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Kern, Wolfgang
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Westers, Theresi ...
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Bellos, Frauke
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Bene, Marie Chri ...
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Bettelheim, Pete ...
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Brodersen, Lisa ...
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show more...
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Burbury, Kate
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Chu, Sung Chao
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Cullen, Matthew
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Porta, Matteo De ...
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Dunlop, Alan Ste ...
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Johansson, Ulrik ...
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Matarraz, Sergio
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Oelschlaegel, Ut ...
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Ogata, Kiyoyuki
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Porwit, Anna
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Preijers, Frank
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Psarra, Katherin ...
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Saft, Leonie
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Subirá, Dolores
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Weiß, Elisabeth
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van der Velden, ...
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van de Loosdrech ...
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Weiss, E
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Hematology
- Articles in the publication
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Cytometry Part B ...
- By the university
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Lund University
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Karolinska Institutet