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Rapid Identificatio...
Rapid Identification of the Tumor-Specific Reactive TIL Repertoire via Combined Detection of CD137, TNF, and IFNγ, Following Recognition of Autologous Tumor-Antigens
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- Draghi, Arianna (author)
- Copenhagen University Hospital
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- Chamberlain, Christopher Aled (author)
- Copenhagen University Hospital
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- Khan, Shawez (author)
- Copenhagen University Hospital
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- Papp, Krisztian (author)
- Eötvös Loránd University
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- Lauss, Martin (author)
- Lund University,Lunds universitet,Melanoma Genomics,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Soraggi, Samuele (author)
- Aarhus University
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- Radic, Haja Dominike (author)
- Copenhagen University Hospital
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- Presti, Mario (author)
- Copenhagen University Hospital
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- Harbst, Katja (author)
- Lund University,Lunds universitet,Lunds Melanomstudiegrupp,Forskargrupper vid Lunds universitet,Melanoma Genomics,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund Melanoma Study Group,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Gokuldass, Aishwarya (author)
- Copenhagen University Hospital
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- Kverneland, Anders (author)
- Copenhagen University Hospital
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- Nielsen, Morten (author)
- Copenhagen University Hospital
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- Westergaard, Marie Christine Wulff (author)
- Copenhagen University Hospital
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- Andersen, Mads Hald (author)
- Copenhagen University Hospital
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- Csabai, Istvan (author)
- Eötvös Loránd University
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- Jönsson, Göran (author)
- Lund University,Lunds universitet,Lunds Melanomstudiegrupp,Forskargrupper vid Lunds universitet,Melanoma Genomics,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund Melanoma Study Group,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Szallasi, Zoltan (author)
- Danish Cancer Society
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- Svane, Inge Marie (author)
- Copenhagen University Hospital
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- Donia, Marco (author)
- Copenhagen University Hospital
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(creator_code:org_t)
- 2021-10-11
- 2021
- English.
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In: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 12
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Abstract
Subject headings
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- Detecting the entire repertoire of tumor-specific reactive tumor-infiltrating lymphocytes (TILs) is essential for investigating their immunological functions in the tumor microenvironment. Current in vitro assays identifying tumor-specific functional activation measure the upregulation of surface molecules, de novo production of antitumor cytokines, or mobilization of cytotoxic granules following recognition of tumor-antigens, yet there is no widely adopted standard method. Here we established an enhanced, yet simple, method for identifying simultaneously CD8+ and CD4+ tumor-specific reactive TILs in vitro, using a combination of widely known and available flow cytometry assays. By combining the detection of intracellular CD137 and de novo production of TNF and IFNγ after recognition of naturally-presented tumor antigens, we demonstrate that a larger fraction of tumor-specific and reactive CD8+ TILs can be detected in vitro compared to commonly used assays. This assay revealed multiple polyfunctionality-based clusters of both CD4+ and CD8+ tumor-specific reactive TILs. In situ, the combined detection of TNFRSF9, TNF, and IFNG identified most of the tumor-specific reactive TIL repertoire. In conclusion, we describe a straightforward method for efficient identification of the tumor-specific reactive TIL repertoire in vitro, which can be rapidly adopted in most cancer immunology laboratories.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- CD137 (4-1BB)
- immune-monitoring
- immune-responses to cancer
- single-cell technologies
- tumor-infiltrating lymphocytes (TILs)
- tumor-specific activation
- tumor-specific reactivity
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Draghi, Arianna
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Chamberlain, Chr ...
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Khan, Shawez
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Papp, Krisztian
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Lauss, Martin
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Soraggi, Samuele
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show more...
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Radic, Haja Domi ...
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Presti, Mario
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Harbst, Katja
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Gokuldass, Aishw ...
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Kverneland, Ande ...
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Nielsen, Morten
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Westergaard, Mar ...
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Andersen, Mads H ...
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Csabai, Istvan
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Jönsson, Göran
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Szallasi, Zoltan
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Svane, Inge Mari ...
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Donia, Marco
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Frontiers in Imm ...
- By the university
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Lund University