SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:lup.lub.lu.se:183dcc6c-c7cb-4ab8-9312-203a9d107a96"
 

Search: onr:"swepub:oai:lup.lub.lu.se:183dcc6c-c7cb-4ab8-9312-203a9d107a96" > Rapid Identificatio...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Rapid Identification of the Tumor-Specific Reactive TIL Repertoire via Combined Detection of CD137, TNF, and IFNγ, Following Recognition of Autologous Tumor-Antigens

Draghi, Arianna (author)
Copenhagen University Hospital
Chamberlain, Christopher Aled (author)
Copenhagen University Hospital
Khan, Shawez (author)
Copenhagen University Hospital
show more...
Papp, Krisztian (author)
Eötvös Loránd University
Lauss, Martin (author)
Lund University,Lunds universitet,Melanoma Genomics,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
Soraggi, Samuele (author)
Aarhus University
Radic, Haja Dominike (author)
Copenhagen University Hospital
Presti, Mario (author)
Copenhagen University Hospital
Harbst, Katja (author)
Lund University,Lunds universitet,Lunds Melanomstudiegrupp,Forskargrupper vid Lunds universitet,Melanoma Genomics,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund Melanoma Study Group,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
Gokuldass, Aishwarya (author)
Copenhagen University Hospital
Kverneland, Anders (author)
Copenhagen University Hospital
Nielsen, Morten (author)
Copenhagen University Hospital
Westergaard, Marie Christine Wulff (author)
Copenhagen University Hospital
Andersen, Mads Hald (author)
Copenhagen University Hospital
Csabai, Istvan (author)
Eötvös Loránd University
Jönsson, Göran (author)
Lund University,Lunds universitet,Lunds Melanomstudiegrupp,Forskargrupper vid Lunds universitet,Melanoma Genomics,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund Melanoma Study Group,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
Szallasi, Zoltan (author)
Danish Cancer Society
Svane, Inge Marie (author)
Copenhagen University Hospital
Donia, Marco (author)
Copenhagen University Hospital
show less...
 (creator_code:org_t)
2021-10-11
2021
English.
In: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 12
Related links:
http://dx.doi.org/10... (free)
show more...
https://www.frontier...
https://lup.lub.lu.s...
https://doi.org/10.3...
show less...
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • Detecting the entire repertoire of tumor-specific reactive tumor-infiltrating lymphocytes (TILs) is essential for investigating their immunological functions in the tumor microenvironment. Current in vitro assays identifying tumor-specific functional activation measure the upregulation of surface molecules, de novo production of antitumor cytokines, or mobilization of cytotoxic granules following recognition of tumor-antigens, yet there is no widely adopted standard method. Here we established an enhanced, yet simple, method for identifying simultaneously CD8+ and CD4+ tumor-specific reactive TILs in vitro, using a combination of widely known and available flow cytometry assays. By combining the detection of intracellular CD137 and de novo production of TNF and IFNγ after recognition of naturally-presented tumor antigens, we demonstrate that a larger fraction of tumor-specific and reactive CD8+ TILs can be detected in vitro compared to commonly used assays. This assay revealed multiple polyfunctionality-based clusters of both CD4+ and CD8+ tumor-specific reactive TILs. In situ, the combined detection of TNFRSF9, TNF, and IFNG identified most of the tumor-specific reactive TIL repertoire. In conclusion, we describe a straightforward method for efficient identification of the tumor-specific reactive TIL repertoire in vitro, which can be rapidly adopted in most cancer immunology laboratories.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Keyword

CD137 (4-1BB)
immune-monitoring
immune-responses to cancer
single-cell technologies
tumor-infiltrating lymphocytes (TILs)
tumor-specific activation
tumor-specific reactivity

Publication and Content Type

art (subject category)
ref (subject category)

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view