onr:"swepub:oai:lup.lub.lu.se:192a79d4-00c5-49cc-ba95-1be0f961067f"
Search: onr:"swepub:oai:lup.lub.lu.se:192a79d4-00c5-49cc-ba95-1be0f961067f" > Cytogenetic and mol...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Jin, YueshengLund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine (author)
Cytogenetic and molecular genetic characterization of immortalized human ovarian surface epithelial cell lines: consistent loss of chromosome 13 and amplification of chromosome 20
- Article/chapterEnglish2004
Publisher, publication year, extent ...
- Elsevier BV,2004
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:192a79d4-00c5-49cc-ba95-1be0f961067f
- https://lup.lub.lu.se/record/286449URI
- https://doi.org/10.1016/j.ygyno.2003.09.007DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Objectives. This study aimed at identifying the genetic events involved in immortalization of ovarian epithelial cells, which might be important steps in ovarian carcinogenesis. Methods. The genetic profiles of five human ovarian surface epithelial (HOSE) cell lines immortalized by retroviral transfection of the human papillomavirus (HPV) E6/E7 genes were thoroughly characterized by chromosome banding and fluorescence in situ hybridization (FISH), at various passages pre- and post-crisis. Results. In pre-crisis, most cells had simple, non-clonal karyotypic changes. Telomere association was the commonest aberration, suggesting that tolermase dysfunction might be an important genetic event leading to cellular crisis. After immortalization post-crisis, however, the karyotypic patterns were non-random. Loss of genetic materials was a characteristic feature. The commonest numerical aberrations were -13, -14, -16, -17, -18, and +5. Among them, loss of chromosome 13 was common change observed in all lines. The only recurrent structural aberration was homogeneously staining regions (hsr) observed in three lines. FISH and combined binary ratio labeling (COBRA)-FISH showed in two cases that the lists were derived from chromosome 20. Clonal evolution was observed in four of the lines. In one line, hsr was the only change shared by all subclones, suggesting that it might be a primary event in cell immortalization. Conclusion. The results of the present study suggested that loss of chromosome 13 and the amplification of chromosome 20 might be early genetic events involved in ovarian cell immortalization, and might be useful targets for the study of genomic aberrations in ovarian carcinogenesis. (C) 2003 Elsevier Inc. All rights reserved.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Medicinsk genetik hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Medical Genetics hsv//eng
- chromosome aberrations
- Hsr
- immortalized ovarian cell lines
Added entries (persons, corporate bodies, meetings, titles ...)
- Zhang, H (author)
- Tsao, SW (author)
- Jin, CharlotteLund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kgen-cji (author)
- Lv, M (author)
- Strömbeck, BodilLund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kgen-bst (author)
- Wiegant, J (author)
- Wan, TSK (author)
- Yuen, PW (author)
- Kwong, YL (author)
- Avdelningen för klinisk genetikInstitutionen för laboratoriemedicin (creator_code:org_t)
Related titles
- In:Gynecologic Oncology: Elsevier BV92:1, s. 183-1911095-68590090-8258
Internet link
Find in a library
- Gynecologic Oncology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Jin, Yuesheng
- Zhang, H
- Tsao, SW
- Jin, Charlotte
- Lv, M
- Strömbeck, Bodil
- show more...
- Wiegant, J
- Wan, TSK
- Yuen, PW
- Kwong, YL
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Medical Genetics
- Articles in the publication
- Gynecologic Onco ...
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
