Non-specific effects of leukotriene synthesis inhibitors on HeLa cell physiology

Sabirsh, A (author)

Bristulf, Jesper (author): Lund University,Lunds universitet,LU Innovation,Sektionen Forskning, samverkan och innovation,Universitetsförvaltningen,Universitetets ledning och förvaltning,Research, Collaboration and Innovation,Central Administration,University Management and Central Administration

Karlsson, Ulf (author): Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine

Owman, Christer (author): Lund University,Lunds universitet,Drug Target Discovery,Forskargrupper vid Lunds universitet,Lund University Research Groups

(creator_code:org_t)

Elsevier BV, 2005
2005
English.
In: Prostaglandins, Leukotrienes and Essential Fatty Acids. - : Elsevier BV. - 0952-3278. ; 73:6, s. 431-440

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Abstract Subject headings

We examined the effects of various leukotriene synthesis inhibitors on calcium signalling in HeLa cells, before and after transfection with BLT1. All of the inhibitors studied were found to reduce increases in intracellular calcium concentration induced by BLT1, but also by an ionophore or activation of various G-protein coupled receptors, regardless of BLT1 expression. In order to explore the mechanism of these apparently general effects we examined HeLa cell expression of leukotriene receptors and biosynthetic enzymes and found that the genes for key leukotriene synthesis enzymes and all of the leukotriene receptors were not expressed. Leukotrienes are involved in the pathology of a variety of cancers, and for HeLa cells leukotrienes have been reported to be important for aspects of the carcinogenic phenotype. We find that leukotriene synthesis inhibitors have non-specific effects, so careful controls are necessary to avoid interpreting non-specific effects as evidence for leukotriene involvement.

By the author/editor: Sabirsh, A; Bristulf, Jesper; Karlsson, Ulf; Owman, Christer; Haeggstrom, JZ

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Neurosciences

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