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Sodium dodecyl sulf...
Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
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- Ramos, Yassel (author)
- Center for Genetic Engineering and Biotechnology (CIGB)
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- González, Annia (author)
- Center for Genetic Engineering and Biotechnology (CIGB)
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- Sosa-Acosta, Patricia (author)
- Center for Genetic Engineering and Biotechnology (CIGB)
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- Perez-Riverol, Yasset (author)
- Wellcome Trust
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- García, Yairet (author)
- Center for Genetic Engineering and Biotechnology (CIGB)
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- Castellanos-Serra, Lila (author)
- Center for Genetic Engineering and Biotechnology (CIGB)
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- Gil, Jeovanis (author)
- Lund University,Lunds universitet,Clinical Protein Science and Imaging,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Sánchez, Aniel (author)
- Lund University,Lunds universitet,Clinical Protein Science and Imaging,Forskargrupper vid Lunds universitet,Lund University Research Groups,Skåne University Hospital
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- González, Luis J. (author)
- Center for Genetic Engineering and Biotechnology (CIGB)
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- Besada, Vladimir (author)
- Center for Genetic Engineering and Biotechnology (CIGB)
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(creator_code:org_t)
- 2019-11-10
- 2019
- English.
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In: Journal of Separation Science. - : Wiley. - 1615-9306 .- 1615-9314. ; 42:24, s. 3712-3717
- Related links:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- electrophoresis
- peptide fractionation
- proteomics
Publication and Content Type
- art (subject category)
- ref (subject category)
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