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Automated microfluidic chromatin immunoprecipitation from 2,000 cells

Wu, Angela R. (author)
Hiatt, Joseph B. (author)
Lu, Rung (author)
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Attema, Joanne (author)
Lund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups
Lobo, Neethan A. (author)
Weissman, Irving L. (author)
Clarke, Michael F. (author)
Quake, Stephen R. (author)
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 (creator_code:org_t)
Royal Society of Chemistry (RSC), 2009
2009
English.
In: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0189 .- 1473-0197. ; 9:10, s. 1365-1370
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, Cumbersome and require a large number of cells, making it difficult to study rare cell types Such its certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, automated fashion while preserving the specificity of the assay. Comparing ChIP results for two modified histone protein targets, we showed our automated microfluidic ChIP (AutoChIP) from 2,000 cells to be comparable to that of conventional ChIP methods using 50,000-500,000 cells. This technology may provide a solution to the need for a high sensitivity, rapid, and automated ChIP assay. and in doing so facilitate the use of ChIP for many interesting and valuable applications.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

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