Search: onr:"swepub:oai:lup.lub.lu.se:22c4a34c-03b3-47a7-8c3c-3db9dbb22d0c" >
Development of algi...
Development of alginate and gelatin-based pleural and tracheal sealants
-
- Gasek, Nathan (author)
- University of Connecticut,University of Vermont
-
- Park, Heon E. (author)
- Canterbury University,University of Vermont
-
- Uriarte, Juan J. (author)
- University of Vermont
-
show more...
-
- Uhl, Franziska E. (author)
- Lund University,Lunds universitet,Vaskulär biologi,Forskargrupper vid Lunds universitet,Vascular Biology,Lund University Research Groups,University of Vermont
-
- Pouliot, Robert A. (author)
- University of Vermont
-
- Riveron, Alexander (author)
- University of Vermont
-
- Moss, Tovah (author)
- University of Vermont
-
- Phillips, Zachary (author)
- University of Vermont
-
- Louie, Jessica (author)
- Lund University
-
- Sharma, Ishna (author)
- University of Toronto
-
- Mohammed, Benefsha (author)
- University of Vermont
-
- Dearborn, Jacob (author)
- University of Vermont
-
- Lee, Patrick C. (author)
- University of Toronto,University of Vermont
-
- Jensen, Todd (author)
- University of Connecticut
-
- Garner, John (author)
- Akina Inc.
-
- Finck, Christine (author)
- University of Connecticut
-
- Weiss, Daniel J. (author)
- University of Vermont
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English 14 s.
-
In: Acta Biomaterialia. - : Elsevier BV. - 1742-7061. ; 131, s. 222-235
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Pleural and tracheal injuries remain significant problems, and an easy to use, effective pleural or tracheal sealant would be a significant advance. The major challenges are requirements for adherence, high strength and elasticity, dynamic durability, appropriate biodegradability, and lack of cell or systemic toxicity. We designed and evaluated two sealant materials comprised respectively of alginate methacrylate and of gelatin methacryloyl, each functionalized by conjugation with dopamine HCl. Both compounds are cross-linked into easily applied as pre-formed hydrogel patches or as in situ hydrogels formed at the wound site utilizing FDA-approved photo-initiators and oxidants. Material testing demonstrates appropriate adhesiveness, tensile strength, burst pressure, and elasticity with no significant cell toxicity in vitro assessments. Air-leak was absent after sealant application to experimentally-induced injuries in ex-vivo rat lung and tracheal models and in ex vivo pig lungs. Sustained repair of experimentally-induced pleural injury was observed for up to one month in vivo rat models and for up to 2 weeks in vivo rat tracheal injury models without obvious air leak or obvious toxicities. The alginate-based sealant worked best in a pre-formed hydrogel patch whereas the gelatin-based sealant worked best in an in situ formed hydrogel at the wound site thus providing two potential approaches. These studies provide a platform for further pre-clinical and potential clinical investigations. Statement of significance: Pneumothorax and pleural effusions resulting from trauma and a range of lung diseases and critical illnesses can result in lung collapse that can be immediately life-threatening or result in chronic leaking (bronchopleural fistula) that is currently difficult to manage. This leads to significantly increased morbidity, mortality, hospital stays, health care costs, and other complications. We have developed sealants originating from alginate and gelatin biomaterials, each functionalized by methacryloylation and by dopamine conjugation to have desired mechanical characteristics for use in pleural and tracheal injuries. The sealants are easily applied, non-cytotoxic, and perform well in vitro and in vivo model systems of lung and tracheal injuries. These initial proof of concept investigations provide a platform for further studies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- Alginate
- Dopamine conjugation
- Gelatin
- Lung
- Methacryloyl
- Pleura
- Sealant
- Trachea
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Gasek, Nathan
-
Park, Heon E.
-
Uriarte, Juan J.
-
Uhl, Franziska E ...
-
Pouliot, Robert ...
-
Riveron, Alexand ...
-
show more...
-
Moss, Tovah
-
Phillips, Zachar ...
-
Louie, Jessica
-
Sharma, Ishna
-
Mohammed, Benefs ...
-
Dearborn, Jacob
-
Lee, Patrick C.
-
Jensen, Todd
-
Garner, John
-
Finck, Christine
-
Weiss, Daniel J.
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Medical Biotechn ...
-
and Biomaterials Sci ...
- Articles in the publication
-
Acta Biomaterial ...
- By the university
-
Lund University