Search: onr:"swepub:oai:lup.lub.lu.se:24060c8a-6945-48a1-976d-8ea0e4e67362" >
Increased beta-cell...
Increased beta-cell volume in mice fed a high-fat diet A dynamic study over 12 months
-
Ahren, Jonatan (author)
-
- Ahrén, Bo (author)
- Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine
-
- Wierup, Nils (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
-
(creator_code:org_t)
- 2014-10-27
- 2010
- English.
-
In: Islets. - : Informa UK Limited. - 1938-2022 .- 1938-2014. ; 2:6, s. 353-356
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://www.tandfonl...
-
http://www.ncbi.nlm....
-
https://lup.lub.lu.s...
-
https://doi.org/10.4...
-
https://lup.lub.lu.s...
-
show less...
Abstract
Subject headings
Close
- As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta-cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive beta-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive beta-cell expansion as evident by early increased islet beta-cell volume and total number of beta-cells, whereas individual beta-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta-cell volume.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- beta cell mass
- insulin resistance
- islet adaptation
- apoptosis
- proliferation
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
-
Islets
(Search for host publication in LIBRIS)
To the university's database