Search: onr:"swepub:oai:lup.lub.lu.se:240c1d0d-8bce-4217-933d-26ad380035f7" >
PARP14 Contributes ...
PARP14 Contributes to the Development of the Tumor-Associated Macrophage Phenotype
-
- Sturniolo, Isotta (author)
- University of Debrecen
-
- Váróczy, Csongor (author)
- University of Debrecen
-
- Regdon, Zsolt (author)
- University of Debrecen
-
show more...
-
- Mázló, Anett (author)
- University of Debrecen
-
- Muzsai, Szabolcs (author)
- University of Debrecen
-
- Bácsi, Attila (author)
- University of Debrecen
-
- Intili, Giorgia (author)
- University of Palermo
-
- Hegedűs, Csaba (author)
- University of Debrecen
-
- Boothby, Mark R. (author)
- Vanderbilt University Medical Center
-
Holechek, Jacob (author)
-
- Ferraris, Dana (author)
- McDaniel College
-
- Schüler, Herwig (author)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
-
- Virág, László (author)
- University of Debrecen
-
show less...
-
(creator_code:org_t)
- 2024
- 2024
- English.
-
In: International Journal of Molecular Sciences. - 1661-6596. ; 25:7
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.3...
-
show less...
Abstract
Subject headings
Close
- Cancers reprogram macrophages (MΦs) to a tumor-growth-promoting TAM (tumor-associated MΦ) phenotype that is similar to the anti-inflammatory M2 phenotype. Poly(ADP-ribose) polymerase (PARP) enzymes regulate various aspects of MΦ biology, but their role in the development of TAM phenotype has not yet been investigated. Here, we show that the multispectral PARP inhibitor (PARPi) PJ34 and the PARP14 specific inhibitor MCD113 suppress the expression of M2 marker genes in IL-4-polarized primary murine MΦs, in THP-1 monocytic human MΦs, and in primary human monocyte-derived MΦs. MΦs isolated from PARP14 knockout mice showed a limited ability to differentiate to M2 cells. In a murine model of TAM polarization (4T1 breast carcinoma cell supernatant transfer to primary MΦs) and in a human TAM model (spheroids formed from JIMT-1 breast carcinoma cells and THP-1-MΦs), both PARPis and the PARP14 KO phenotype caused weaker TAM polarization. Increased JIMT-1 cell apoptosis in co-culture spheroids treated with PARPis suggested reduced functional TAM reprogramming. Protein profiling arrays identified lipocalin-2, macrophage migration inhibitory factor, and plasminogen activator inhibitor-1 as potential (ADP-ribosyl)ation-dependent mediators of TAM differentiation. Our data suggest that PARP14 inhibition might be a viable anticancer strategy with a potential to boost anticancer immune responses by reprogramming TAMs.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- ADP-ribosylation
- breast cancer
- macrophage
- PARP14
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Sturniolo, Isott ...
-
Váróczy, Csongor
-
Regdon, Zsolt
-
Mázló, Anett
-
Muzsai, Szabolcs
-
Bácsi, Attila
-
show more...
-
Intili, Giorgia
-
Hegedűs, Csaba
-
Boothby, Mark R.
-
Holechek, Jacob
-
Ferraris, Dana
-
Schüler, Herwig
-
Virág, László
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cancer and Oncol ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Immunology in th ...
- Articles in the publication
-
International Jo ...
- By the university
-
Lund University