Search: onr:"swepub:oai:lup.lub.lu.se:2465df78-5ac8-4039-a7c3-ec4388391f53" >
Visualizing the ent...
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Freitag, C.Gothenburg University,University of Gothenburg,Lund University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
(author)
Visualizing the entire DNA from a chromosome in a single frame
- Article/chapterEnglish2015
Publisher, publication year, extent ...
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LIBRIS-ID:oai:lup.lub.lu.se:2465df78-5ac8-4039-a7c3-ec4388391f53
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https://lup.lub.lu.se/record/7969174URI
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https://doi.org/10.1063/1.4923262DOI
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https://research.chalmers.se/publication/222738URI
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https://gup.ub.gu.se/publication/222738URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-263040URI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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The contiguity and phase of sequence information are intrinsic to obtain complete understanding of the genome and its relationship to phenotype. We report the fabrication and application of a novel nanochannel design that folds megabase lengths of genomic DNA into a systematic back-and-forth meandering path. Such meandering nanochannels enabled us to visualize the complete 5.7 Mbp (1mm) stained DNA length of a Schizosaccharomyces pombe chromosome in a single frame of a CCD. We were able to hold the DNA in situ while implementing partial denaturation to obtain a barcode pattern that we could match to a reference map using the Poland-Scheraga model for DNA melting. The facility to compose such long linear lengths of genomic DNA in one field of view enabled us to directly visualize a repeat motif, count the repeat unit number, and chart its location in the genome by reference to unique barcode motifs found at measurable distances from the repeat. Meandering nanochannel dimensions can easily be tailored to human chromosome scales, which would enable the whole genome to be visualized in seconds. (C) 2015 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Noble, CharlestonLund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science(Swepub:lu)thep-can
(author)
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Fritzsche, Joachim,1977Gothenburg University,University of Gothenburg,Chalmers University of Technology,Göteborgs universitet,Chalmers tekniska högskola,Institutionen för fysik (GU),Department of Physics (GU)(Swepub:cth)joafri
(author)
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Persson, Fredrik,1979Uppsala universitet,Gothenburg University,Uppsala University,University of Gothenburg,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),Beräknings- och systembiologi(Swepub:gu)xpefre
(author)
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Reiter-Schad, MichaelaLund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science(Swepub:lu)thep-mls
(author)
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Nilsson, AdamLund University,Lunds universitet,Atomfysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Atomic Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)fysi-adn
(author)
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Graneli, Annette,1973Gothenburg University,University of Gothenburg,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)(Swepub:gu)xgrane
(author)
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Ambjörnsson, TobiasLund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science(Swepub:lu)thep-tar
(author)
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Mir, K. U.University of Oxford,Harvard University,Wellcome Trust Centre for Human Genetics
(author)
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Tegenfeldt, JonasGothenburg University,University of Gothenburg,Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)(Swepub:gu)xjonte
(author)
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University of GothenburgLund University
(creator_code:org_t)
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In:Biomicrofluidics: AIP Publishing9:41932-1058
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- NATURAL SCIENCES
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Biomicrofluidics
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Lund University
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Chalmers University of Technology
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