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Ezrin is a novel pr...
Ezrin is a novel protein partner of aquaporin-5 in human salivary glands and shows altered expression and cellular localization in sjögren’s syndrome
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- Chivasso, Clara (author)
- Université Libre de Bruxelles (ULB)
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- Hagströmer, Carl Johan (author)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Rose, Kristie L. (author)
- Vanderbilt University
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- Lhotellerie, Florent (author)
- Université Libre de Bruxelles (ULB)
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- Leblanc, Lionel (author)
- Université Libre de Bruxelles (ULB)
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- Wang, Zhen (author)
- Vanderbilt University
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- Moscato, Stefania (author)
- University of Pisa
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Chevalier, Clément (author)
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Zindy, Egor (author)
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- Martin, Maud (author)
- Université Libre de Bruxelles (ULB)
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- Vanhollebeke, Benoit (author)
- Université Libre de Bruxelles (ULB)
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- Gregoire, Françoise (author)
- Université Libre de Bruxelles (ULB)
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- Bolaky, Nargis (author)
- Université Libre de Bruxelles (ULB)
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- Perret, Jason (author)
- Université Libre de Bruxelles (ULB)
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- Baldini, Chiara (author)
- University of Pisa
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- Soyfoo, Muhammad Shahnawaz (author)
- Université Libre de Bruxelles (ULB)
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- Mattii, Letizia (author)
- University of Pisa
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- Schey, Kevin L. (author)
- Vanderbilt University
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- Törnroth-Horsefield, Susanna (author)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Delporte, Christine (author)
- Université Libre de Bruxelles (ULB)
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(creator_code:org_t)
- 2021-08-26
- 2021
- English.
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In: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 22:17
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Abstract
Subject headings
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- Sjögren’s syndrome (SS) is an exocrinopathy characterized by the hypofunction of salivary glands (SGs). Aquaporin-5 (AQP5); a water channel involved in saliva formation; is aberrantly dis-tributed in SS SG acini and contributes to glandular dysfunction. We aimed to investigate the role of ezrin in AQP5 mislocalization in SS SGs. The AQP5–ezrin interaction was assessed by immuno-precipitation and proteome analysis and by proximity ligation assay in immortalized human SG cells. We demonstrated, for the first time, an interaction between ezrin and AQP5. A model of the complex was derived by computer modeling and in silico docking; suggesting that AQP5 interacts with the ezrin FERM-domain via its C-terminus. The interaction was also investigated in human minor salivary gland (hMSG) acini from SS patients (SICCA-SS); showing that AQP5–ezrin complexes were absent or mislocalized to the basolateral side of SG acini rather than the apical region compared to controls (SICCA-NS). Furthermore, in SICCA-SS hMSG acinar cells, ezrin immunore-activity was decreased at the acinar apical region and higher at basal or lateral regions, accounting for altered AQP5–ezrin co-localization. Our data reveal that AQP5–ezrin interactions in human SGs could be involved in the regulation of AQP5 trafficking and may contribute to AQP5-altered localization in SS patients.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Aquaporin-5
- Ezrin
- Protein–protein interaction
- Salivary glands
- Sjögren’s syndrome
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Chivasso, Clara
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Hagströmer, Carl ...
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Rose, Kristie L.
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Lhotellerie, Flo ...
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Leblanc, Lionel
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Wang, Zhen
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show more...
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Moscato, Stefani ...
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Chevalier, Cléme ...
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Zindy, Egor
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Martin, Maud
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Vanhollebeke, Be ...
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Gregoire, Franço ...
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Bolaky, Nargis
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Perret, Jason
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Baldini, Chiara
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Soyfoo, Muhammad ...
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Mattii, Letizia
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Schey, Kevin L.
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Törnroth-Horsefi ...
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Delporte, Christ ...
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