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Induction of human ...
Induction of human hemogenesis in adult fibroblasts by defined factors and hematopoietic coculture
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- Daniel, Michael G. (author)
- Icahn School of Medicine at Mount Sinai
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- Sachs, David (author)
- Icahn School of Medicine at Mount Sinai
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- Bernitz, Jeffrey M. (author)
- ETH Zürich
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- Fstkchyan, Yesai (author)
- Icahn School of Medicine at Mount Sinai
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- Rapp, Katrina (author)
- Icahn School of Medicine at Mount Sinai
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- Satija, Namita (author)
- Icahn School of Medicine at Mount Sinai
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- Law, Kenneth (author)
- Rocket Pharmaceuticals Ltd
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- Patel, Foram (author)
- Icahn School of Medicine at Mount Sinai
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- Gomes, Andreia M. (author)
- University of Coimbra
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- Kim, Huen Suk (author)
- Icahn School of Medicine at Mount Sinai
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- Pereira, Carlos Filipe (author)
- Lund University,Lunds universitet,Cellulär omprogrammering i hematopoes och immunitet,Forskargrupper vid Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,Cell Reprogramming in Hematopoiesis and Immunity,Lund University Research Groups,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine
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- Chen, Benjamin (author)
- Icahn School of Medicine at Mount Sinai
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- Lemischka, Ihor R. (author)
- Icahn School of Medicine at Mount Sinai
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- Moore, Kateri A. (author)
- Icahn School of Medicine at Mount Sinai
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(creator_code:org_t)
- 2019-10-12
- 2019
- English.
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In: FEBS Letters. - : Wiley. - 0014-5793 .- 1873-3468. ; 593:23, s. 3266-3287
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Abstract
Subject headings
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- Transcription factor (TF)-based reprogramming of somatic tissues holds great promise for regenerative medicine. Previously, we demonstrated that the TFs GATA2, GFI1B, and FOS convert mouse and human fibroblasts to hemogenic endothelial-like precursors that generate hematopoietic stem progenitor (HSPC)-like cells over time. This conversion is lacking in robustness both in yield and biological function. Herein, we show that inclusion of GFI1 to the reprogramming cocktail significantly expands the HSPC-like population. AFT024 coculture imparts functional potential to these cells and allows quantification of stem cell frequency. Altogether, we demonstrate an improved human hemogenic induction protocol that could provide a valuable human in vitro model of hematopoiesis for disease modeling and a platform for cell-based therapeutics. Database: Gene expression data are available in the Gene Expression Omnibus (GEO) database under the accession number GSE130361.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- AFT024
- coculture
- hematopoietic stem cells
- reprogramming
- transcription factors
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Daniel, Michael ...
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Sachs, David
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Bernitz, Jeffrey ...
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Fstkchyan, Yesai
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Rapp, Katrina
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Satija, Namita
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show more...
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Law, Kenneth
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Patel, Foram
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Gomes, Andreia M ...
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Kim, Huen Suk
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Pereira, Carlos ...
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Chen, Benjamin
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Lemischka, Ihor ...
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Moore, Kateri A.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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FEBS Letters
- By the university
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Lund University