onr:"swepub:oai:lup.lub.lu.se:2bdbe307-ebff-4372-9c03-470aab60f480"
Search: onr:"swepub:oai:lup.lub.lu.se:2bdbe307-ebff-4372-9c03-470aab60f480" > Improved porous sil...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Lee, SangWook (author)
Improved porous silicon microarray based prostate specific antigen immunoassay by optimized surface density of the capture antibody
- Article/chapterEnglish2013
Publisher, publication year, extent ...
- Elsevier BV,2013
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:2bdbe307-ebff-4372-9c03-470aab60f480
- https://lup.lub.lu.se/record/4098911URI
- https://doi.org/10.1016/j.aca.2013.06.041DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We further investigated PSA spiked into human female serum in the range of 800 fg mL(-1) to 500 ng mL(-1). The microarray showed a LOD of 800 fg mL(-1) and a dynamic range of 800 fg mL(-1) to 80 ng mL(-1) in serum spiked samples. (C) 2013 Elsevier B.V. All rights reserved.
Subject headings and genre
- NATURVETENSKAP Kemi Analytisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
- Antibody microarray
- Porous silicon
- Sandwich immunoassay
- Prostate
- specific antigen
Added entries (persons, corporate bodies, meetings, titles ...)
- Kim, Soyoun (author)
- Malm, JohanLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-jma (author)
- Jeong, Ok Chan (author)
- Lilja, HansLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-hli (author)
- Laurell, ThomasLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-tla (author)
- Klinisk kemi, MalmöForskargrupper vid Lunds universitet (creator_code:org_t)
Related titles
- In:Analytica Chimica Acta: Elsevier BV796, s. 108-1141873-43240003-2670
Internet link
Find in a library
- Analytica Chimica Acta (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Lee, SangWook
- Kim, Soyoun
- Malm, Johan
- Jeong, Ok Chan
- Lilja, Hans
- Laurell, Thomas
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- and Analytical Chemi ...
- Articles in the publication
- Analytica Chimic ...
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
