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DNA Extraction Prot...
DNA Extraction Protocols for Whole-Genome Sequencing in Marine Organisms
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- Panova, M (author)
- University of Gothenburg
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- Aronsson, H (author)
- University of Gothenburg
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Cameron, RA (author)
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- Dahl, P (author)
- University of Gothenburg
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- Godhe, A (author)
- University of Gothenburg
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- Lind, U (author)
- University of Gothenburg
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- Ortega-Martinez, O (author)
- University of Gothenburg
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- Pereyra, R (author)
- University of Gothenburg
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- Tesson, Sylvie (author)
- Lund University,Lunds universitet,Enhet akvatisk ekologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Division aquatic ecology,Department of Biology,Faculty of Science
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- Wrange, A-L (author)
- University of Gothenburg
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- Blomberg, A (author)
- University of Gothenburg
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- Johannesson, K (author)
- University of Gothenburg
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Bourlat, Sarah (editor)
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(creator_code:org_t)
- New York, NY : Springer New York, 2016
- 2016
- English.
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In: Marine Genomics - Methods and Protocols. - New York, NY : Springer New York. - 1064-3745. - 9781493937745 - 9781493937721 ; 1452, s. 13-44
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http://dx.doi.org/10...
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Abstract
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- The marine environment harbors a large proportion of the total biodiversity on this planet, including the majority of the earths’ different phyla and classes. Studying the genomes of marine organisms can bring interesting insights into genome evolution. Today, almost all marine organismal groups are understudied with respect to their genomes. One potential reason is that extraction of high-quality DNA in sufficient amounts is challenging for many marine species. This is due to high polysaccharide content, polyphenols and other secondary metabolites that will inhibit downstream DNA library preparations. Consequently, protocols developed for vertebrates and plants do not always perform well for invertebrates and algae. In addition, many marine species have large population sizes and, as a consequence, highly variable genomes. Thus, to facilitate the sequence read assembly process during genome sequencing, it is desirable to obtain enough DNA from a single individual, which is a challenge in many species of invertebrates and algae. Here, we present DNA extraction protocols for seven marine species (four invertebrates, two algae, and a marine yeast), optimized to provide sufficient DNA quality and yield for de novo genome sequencing projects.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Publication and Content Type
- kap (subject category)
- ref (subject category)
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- By the author/editor
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Panova, M
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Aronsson, H
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Cameron, RA
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Dahl, P
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Godhe, A
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Lind, U
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show more...
-
Ortega-Martinez, ...
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Pereyra, R
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Tesson, Sylvie
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Wrange, A-L
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Blomberg, A
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Johannesson, K
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Bourlat, Sarah
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
- Articles in the publication
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Marine Genomics ...
- By the university
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Lund University