Factor VIIa binding to endothelial cell protein C receptor: Differences between mouse and human systems

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:lup.lub.lu.se:310f6818-e4b4-49b7-9761-a6d6c913e709" > Factor VIIa binding...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Sen, Prosenjit (author)

Factor VIIa binding to endothelial cell protein C receptor: Differences between mouse and human systems

Article/chapterEnglish2012

Publisher, publication year, extent ...

2012

Numbers

LIBRIS-ID:oai:lup.lub.lu.se:310f6818-e4b4-49b7-9761-a6d6c913e709
https://lup.lub.lu.se/record/2911678URI
https://doi.org/10.1160/TH11-09-0672DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:art swepub-publicationtype
Subject category:ref swepub-contenttype

Notes

Recent in vitro studies have shown that the zymogen and activated form of factor (F)VII bind to endothelial cell protein C receptor (EPCR). At present, there is no evidence that FVIIa binds to EPCR on vascular endothelium in vivo in the presence of circulating protein C, a primary ligand for EPCR. The present study was carried out to investigate the interaction of murine and human ligands with murine EPCR both in vivo and in vitro. Measurement of endogenous plasma levels of FVII in wild-type, EPCR-deficient and EPCR-over expressing mice showed slightly lower levels of FVII in EPCR-over expressing mice. However, infusion of high concentrations of competing ligands, either human APCi or FVIIai, to EPCR-over expressing mice failed to increase plasma levels of mouse FVII whereas they increased the plasma levels of protein C by two- to three-fold. Examining the association of exogenously administered mouse FVIIa or human FVIIa by immunohistochemistry revealed that human, but not murine FVIIa, binds to the murine endothelium in an EPCR-dependent manner. vitro binding studies performed using surface plasmon resonance and endothelial cells revealed that murine FVIIa binds murine EPCR negligibly. Human FVIIa binding to EPCR, particularly to mouse EPCR, is markedly enhanced by availability of Mg2+ ions. In summary, our data show that murine FVIIa binds poorly to murine EPCR, whereas human FVIIa binds efficiently to both murine and human EPCR. Our data suggest that one should consider the use of human FVIIa in mouse models to investigate the significance of FVIIa and EPCR interaction.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Kardiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Cardiac and Cardiovascular Systems hsv//eng
Endothelial cell protein C receptor
factor VIIa
protein C

Added entries (persons, corporate bodies, meetings, titles ...)

Clark, Curtis A. (author)
Gopalakrishnan, Ramakrishnan (author)
Hedner, UllaLund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Department of Clinical Sciences, Malmö,Faculty of Medicine(Swepub:lu)medf-uhe (author)
Esmon, Charles T. (author)
Pendurthi, Usha R. (author)
Rao, L. Vijaya Mohan (author)
Institutionen för kliniska vetenskaper, MalmöMedicinska fakulteten (creator_code:org_t)

Related titles

In:Thrombosis and Haemostasis107:5, s. 951-9610340-6245

Internet link

Find in a library

Thrombosis and Haemostasis (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Sen, Prosenjit; Clark, Curtis A.; Gopalakrishnan, ...; Hedner, Ulla; Esmon, Charles T ...; Pendurthi, Usha ...; show more...; Rao, L. Vijaya M ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cardiac and Card ...

Articles in the publication: Thrombosis and H ...

By the university: Lund University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se