Search: onr:"swepub:oai:lup.lub.lu.se:31619e9f-1802-48fe-a92a-96f6270f66ab" >
Combined Experiment...
Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis
-
- Kutsche, Lisa K. (author)
- Dresden University of Technology
-
- Gysi, Deisy M. (author)
- Free University of Berlin,Leipzig University
-
- Fallmann, Joerg (author)
- Leipzig University
-
show more...
-
- Lenk, Kerstin (author)
- Dresden University of Technology
-
- Petri, Rebecca (author)
- Lund University,Lunds universitet,Molekylär neurogenetik,Forskargrupper vid Lunds universitet,Molecular Neurogenetics,Lund University Research Groups
-
- Swiersy, Anka (author)
- Dresden University of Technology
-
- Klapper, Simon D. (author)
- Dresden University of Technology
-
- Pircs, Karolina (author)
- Lund University,Lunds universitet,Molekylär neurogenetik,Forskargrupper vid Lunds universitet,Molecular Neurogenetics,Lund University Research Groups
-
- Khattak, Shahryar (author)
- Dresden University of Technology
-
- Stadler, Peter F. (author)
- Max Planck Institute for Mathematics in the Sciences,Leipzig University,Santa Fe Institute
-
- Jakobsson, Johan (author)
- Lund University,Lunds universitet,Molekylär neurogenetik,Forskargrupper vid Lunds universitet,Molecular Neurogenetics,Lund University Research Groups
-
- Nowick, Katja (author)
- Free University of Berlin
-
- Busskamp, Volker (author)
- Dresden University of Technology
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2018
- 2018
- English 15 s.
-
In: Cell systems. - : Elsevier BV. - 2405-4712. ; 7:4, s. 438-452
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
http://www.cell.com/...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Non-coding RNAs regulate many biological processes including neurogenesis. The brain-enriched miR-124 has been assigned as a key player of neuronal differentiation via its complex but little understood regulation of thousands of annotated targets. To systematically chart its regulatory functions, we used CRISPR/Cas9 gene editing to disrupt all six miR-124 alleles in human induced pluripotent stem cells. Upon neuronal induction, miR-124-deleted cells underwent neurogenesis and became functional neurons, albeit with altered morphology and neurotransmitter specification. Using RNA-induced-silencing-complex precipitation, we identified 98 high-confidence miR-124 targets, of which some directly led to decreased viability. By performing advanced transcription-factor-network analysis, we identified indirect miR-124 effects on apoptosis, neuronal subtype differentiation, and the regulation of previously uncharacterized zinc finger transcription factors. Our data emphasize the need for combined experimental- and system-level analyses to comprehensively disentangle and reveal miRNA functions, including their involvement in the neurogenesis of diverse neuronal cell types found in the human brain.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- AGO2-RIP-seq
- gene regulatory network analysis
- miR-124 targetome
- miRNA dynamics
- miRNA regulation
- miRNA-transcription factor networks
- neuronal differentiation from human stem cells
- neuronal miRNAs
- systems biology
- ZNF787
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Kutsche, Lisa K.
-
Gysi, Deisy M.
-
Fallmann, Joerg
-
Lenk, Kerstin
-
Petri, Rebecca
-
Swiersy, Anka
-
show more...
-
Klapper, Simon D ...
-
Pircs, Karolina
-
Khattak, Shahrya ...
-
Stadler, Peter F ...
-
Jakobsson, Johan
-
Nowick, Katja
-
Busskamp, Volker
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Neurosciences
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Cell systems
- By the university
-
Lund University