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Calorimetric analysis of cephalosporins using an immobilized TEM-1 beta-lactamase on Ni2+ chelating sepharose fast flow
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- Lawung, R (author)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Danielsson, Bengt (author)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Prachayasittikul, V (author)
- Mahidol University
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- (creator_code:org_t)
- Elsevier BV, 2001
- 2001
- English 6 s.
- In: Analytical Biochemistry. - : Elsevier BV. - 0003-2697. ; 296:1, s. 57-62
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Two beta-lactamases, penicillinase type I from Bacillus cereus and TEM-1 beta-lactamase from Haemophilus ducreyi, were immobilized on a Chelating Sepharose Fast Flow column loaded with Ni2+ in an active form. Flow-injection analysis of beta-lactams was performed by using an enzyme column reactor fitted into the enzyme thermistor. With both enzymes it was possible to monitor both penicillins and cephalosporins. Moreover, Michaelis constants of the TEM-1 beta-lactamase were markedly increased upon immobilization for all substrates, especially carbenicillin, cephaloridine, and cefoperazone.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Anti-Bacterial Agents
- Bacillus cereus
- Calorimetry
- Carbenicillin
- Cefoperazone
- Cephaloridine
- Cephalosporins
- Chelating Agents
- Chromatography, Affinity
- Chromatography, Agarose
- Enzymes, Immobilized
- Haemophilus ducreyi
- Nickel
- Penicillinase
- Penicillins
- beta-Lactamases
- beta-Lactams
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- art (subject category)
- ref (subject category)
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