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- Andersson, TildeLund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups (author)
Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum
- Article/chapterEnglish2020
Publisher, publication year, extent ...
- 2020-11-20
- Frontiers Media SA,2020
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:32ae4433-593f-4fce-b497-44204f0fcb25
- https://lup.lub.lu.se/record/32ae4433-593f-4fce-b497-44204f0fcb25URI
- https://doi.org/10.3389/fcimb.2020.571578DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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- Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti α-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from S. aureus infections where α-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, α-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized α-hemolysin with high affinity (KD = 2.75 x 10-7 M) and demonstrated a statistically significant difference (p < 0.0001) between the α-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Infektionsmedicin hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Infectious Medicine hsv//eng
- alpha hemolysin
- molecular imprinting
- sepsis
- SPR
- Staphylococcus aureus
Added entries (persons, corporate bodies, meetings, titles ...)
- Bläckberg, AnnaLund University,Lunds universitet,Translationell infektionsmedicin,Forskargrupper vid Lunds universitet,Translational infection medicine,Lund University Research Groups,Skåne University Hospital(Swepub:lu)an7442bl (author)
- Lood, RolfLund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups(Swepub:lu)med-rll (author)
- Ertürk Bergdahl, GizemLund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups(Swepub:lu)gi2856er (author)
- Experimental Infection MedicineForskargrupper vid Lunds universitet (creator_code:org_t)
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- In:Frontiers in cellular and infection microbiology: Frontiers Media SA102235-2988
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