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Cloning and functio...
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Sharma, PUppsala University
(author)
Cloning and functional expression of the guinea pig neuropeptide Y Y2 receptor
- Article/chapterEnglish1998
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Numbers
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LIBRIS-ID:oai:lup.lub.lu.se:359471b0-6afa-428b-b61e-b387d146b9e5
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https://lup.lub.lu.se/record/359471b0-6afa-428b-b61e-b387d146b9e5URI
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https://doi.org/10.1016/s0167-0115(98)00049-4DOI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Five neuropeptide Y (NPY) receptor subtypes have been cloned in mammals. The degree of sequence conservation differs considerably between subtypes as well as between evolutionary lineages. To shed further light on this, we have cloned the five NPY receptors in the guinea pig. Here, we report the cloning of the guinea pig Y2 receptor. The Y2 receptor is generally highly conserved, with 90-95% identity between different orders of mammals, including the guinea pig. The guinea pig receptor has a divergent cytoplasmic tail, indicating possible differences in regulation of signalling and/or down regulation. COS-7 cells transiently transfected with the gpY2 receptor show saturable 125I-PYY binding with a Kd = 6 pM. In displacement experiments, the gpY2 receptor was similar to the human and rat receptors with the following rank order of potencies: pNPY > pPYY > pNPY13-36 = pNPY22-36 > [Leu31Pro34]NPY > BIBP3226. Thus, the guinea pig Y2 receptor is well conserved in comparison with human and rat with regard to both amino acid sequence and pharmacological profile.
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Holmberg, S KUppsala University
(author)
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Eriksson, HUppsala University
(author)
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Beck-Sickinger, A GETH Zürich
(author)
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Grundemar, LLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)med-lgd
(author)
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Larhammar, DUppsala University
(author)
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Uppsala UniversityETH Zürich
(creator_code:org_t)
Related titles
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In:Regulatory Peptides: Elsevier BV75-76, s. 23-280167-0115
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