SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:lup.lub.lu.se:35ac359d-bd09-4b43-a41b-af57403f3534"
 

Search: onr:"swepub:oai:lup.lub.lu.se:35ac359d-bd09-4b43-a41b-af57403f3534" > Invisible detergent...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Midtgaard, Søren Roi (author)

Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering

  • Article/chapterEnglish2018

Publisher, publication year, extent ...

  • 2017-12-30
  • Wiley,2018

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:35ac359d-bd09-4b43-a41b-af57403f3534
  • https://lup.lub.lu.se/record/35ac359d-bd09-4b43-a41b-af57403f3534URI
  • https://doi.org/10.1111/febs.14345DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Darwish, Tamim A (author)
  • Pedersen, Martin Cramer (author)
  • Huda, Pie (author)
  • Larsen, Andreas Haahr (author)
  • Jensen, Grethe Vestergaard (author)
  • Kynde, Søren Andreas Røssel (author)
  • Skar-Gislinge, Nicholas (author)
  • Nielsen, Agnieszka Janina Zygadlo (author)
  • Olesen, Claus (author)
  • Blaise, Mickael (author)
  • Dorosz, Jerzy Józef (author)
  • Thorsen, Thor Seneca (author)
  • Venskutonytė, RamintaLund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups(Swepub:lu)ra5746ve (author)
  • Krintel, ChristianUniversity of Copenhagen(Swepub:lu)med-ctk (author)
  • Møller, Jesper V (author)
  • Frielinghaus, Henrich (author)
  • Gilbert, Elliot Paul (author)
  • Martel, Anne (author)
  • Kastrup, Jette Sandholm (author)
  • Jensen, Poul Erik (author)
  • Nissen, Poul (author)
  • Arleth, Lise (author)
  • Medicinsk strukturbiologiForskargrupper vid Lunds universitet (creator_code:org_t)

Related titles

  • In:The FEBS Journal: Wiley285:2, s. 357-3711742-464X1742-4658

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view