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Invisible detergent...
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Midtgaard, Søren Roi
(author)
Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering
- Article/chapterEnglish2018
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LIBRIS-ID:oai:lup.lub.lu.se:35ac359d-bd09-4b43-a41b-af57403f3534
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https://lup.lub.lu.se/record/35ac359d-bd09-4b43-a41b-af57403f3534URI
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https://doi.org/10.1111/febs.14345DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.
Added entries (persons, corporate bodies, meetings, titles ...)
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Darwish, Tamim A
(author)
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Pedersen, Martin Cramer
(author)
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Huda, Pie
(author)
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Larsen, Andreas Haahr
(author)
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Jensen, Grethe Vestergaard
(author)
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Kynde, Søren Andreas Røssel
(author)
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Skar-Gislinge, Nicholas
(author)
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Nielsen, Agnieszka Janina Zygadlo
(author)
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Olesen, Claus
(author)
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Blaise, Mickael
(author)
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Dorosz, Jerzy Józef
(author)
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Thorsen, Thor Seneca
(author)
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Venskutonytė, RamintaLund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups(Swepub:lu)ra5746ve
(author)
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Krintel, ChristianUniversity of Copenhagen(Swepub:lu)med-ctk
(author)
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Møller, Jesper V
(author)
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Frielinghaus, Henrich
(author)
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Gilbert, Elliot Paul
(author)
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Martel, Anne
(author)
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Kastrup, Jette Sandholm
(author)
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Jensen, Poul Erik
(author)
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Nissen, Poul
(author)
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Arleth, Lise
(author)
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Medicinsk strukturbiologiForskargrupper vid Lunds universitet
(creator_code:org_t)
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In:The FEBS Journal: Wiley285:2, s. 357-3711742-464X1742-4658
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Darwish, Tamim A
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Pedersen, Martin ...
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Huda, Pie
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Larsen, Andreas ...
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Jensen, Grethe V ...
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Kynde, Søren And ...
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Skar-Gislinge, N ...
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Nielsen, Agniesz ...
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Olesen, Claus
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Dorosz, Jerzy Jó ...
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Thorsen, Thor Se ...
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Venskutonytė, Ra ...
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Krintel, Christi ...
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Møller, Jesper V
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Frielinghaus, He ...
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Gilbert, Elliot ...
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Martel, Anne
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Kastrup, Jette S ...
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