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Deep sequencing rev...
Deep sequencing reveals global patterns of mRNA recruitment during translation initiation
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- Gao, Rong (author)
- Peking University
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- Yu, Kai (author)
- Peking University
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- Nie, Jukui (author)
- Peking University
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- Lian, Tengfei (author)
- Peking University
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- Jin, Jianshi (author)
- Peking University
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- Liljas, Anders (author)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Su, Xiao Dong (author)
- Peking University
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(creator_code:org_t)
- 2016-07-27
- 2016
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
- Related links:
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Abstract
Subject headings
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- In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation region (TIR), including the S1 binding sequence, the Shine-Dalgarno (SD)/anti-SD interaction and the second codon, at the equilibrium of different initiation complexes. Moreover, our experiments reveal the conformational changes and regional dynamics throughout the dynamic process of mRNA recruitment.
Subject headings
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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