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Loss- and gain-of-f...
Loss- and gain-of-function analyses reveal targets of Pax6 in the developing mouse telencephalon.
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- Holm, Pontus (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Institute for Stem Cell Research, National Research Center for Environment and Health, Neuherberg/Munich
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- Mader, Michael T (author)
- Institute for Stem Cell Research, National Research Center for Environment and Health, Neuherberg/Munich
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- Haubst, Nicole (author)
- Institute for Stem Cell Research, National Research Center for Environment and Health, Neuherberg/Munich
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- Wizenmann, Andrea (author)
- Institute for Stem Cell Research, National Research Center for Environment and Health, Neuherberg/Munich
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- Sigvardsson, Mikael (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine
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- Gotz, Magdalena (author)
- Institute for Stem Cell Research, National Research Center for Environment and Health, Neuherberg/Munich
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
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In: Molecular and Cellular Neuroscience. - : Elsevier BV. - 1044-7431 .- 1095-9327. ; 34:1, s. 99-119
- Related links:
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http://www.ncbi.nlm....
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Appropriate neurogenesis and patterning of the forebrain requires the transcription factor Pax6, yet it is largely unknown how Pax6 exerts its effects at the molecular level. To characterize Pax6-mediated regulation of gene expression during murine forebrain neurogenesis, we performed microarray analysis with tissue from the dorsal Pax6-dependent telencephalon and the ventral Pax6-negative telencephalon at the onset of neurogenesis (E12) and at mid-neurogenesis (E15) in wild-type and Pax6-deficient mutant littermates. In the Pax6-deficient cortex the expression levels of various transcription factors involved in neurogenesis (like Satb2, Nfia, AP-2 gamma, NeuroD6, Ngn2, Tbr2, Bhlhb5) and the retinoic acid signalling molecule Rlbp1 were reduced. Regulation by Pax6 could be confirmed upon electroporation of a Pax6- and a dominant-negative Pax6-containing vector into embryonic cortex. Taken together, our data reveal novel insights into the molecular pathways regulated by Pax6 during cortical neurogenesis. Most intriguingly, this analysis revealed time- and region-specific differences in Pax6-mediated transcription, explaining the specific function of Pax6 at early and later stages of neurogenesis. (c) 2006 Elsevier Inc. All rights reserved.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- neurogenesis
- retinoic acid signalling
- electroporation
- cortex
- Pax6
- microarray
- telencephalon
- ganglionic eminence
- small eye
- Pax6; cortex; ganglionic eminence; small eye; telencephalon; microarray; electroporation; neurogenesis; retinoic acid signalling
- TECHNOLOGY
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