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Oxidative stress, m...
Oxidative stress, mitochondrial permeability transition and activation of caspases in calcium ionophore A23187-induced death of cultured striatal neurons
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- Petersén, Åsa (author)
- Lund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine
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- Castilho, Roger F. (author)
- Lund University
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- Hansson, Oskar (author)
- Lund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine
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- Wieloch, Tadeusz (author)
- Lund University,Lunds universitet,Laboratory for Experimental Brain Research,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Brundin, Patrik (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
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(creator_code:org_t)
- 2000
- 2000
- English 10 s.
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In: Brain Research. - 0006-8993. ; 857:1-2, s. 20-29
- Related links:
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http://dx.doi.org/10...
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Abstract
Subject headings
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- Disruption of intracellular calcium homeostasis is thought to play a role in neurodegenerative disorders such as Huntington's disease (HD). To study different aspects of putative pathogenic mechanisms in HD, we aimed to establish an in vitro model of calcium-induced toxicity in striatal neurons. The calcium ionophore A23187 induced a concentration- and time-dependent cell death in cultures of embryonic striatal neurons, causing both apoptosis and necrosis. Cell death was significantly reduced by the cell-permeant antioxidant manganese(III)tetrakis(4-benzoic acid) porphyrin (MnTBAP). Cyclosporin A and its analogue N-MeVal-4-cyclosporin also reduced the incidence of cell death, suggesting the participation of mitochondrial permeability transition in this process. Furthermore, addition of either of two types of caspase inhibitors, Ac-YVAD-CHO (acetyl-Tyr-Val-Ala-Asp-aldehyde) and Ac-DEVD-CHO (acetyl-Asp-Glu-Val-Asp-aldehyde), to the striatal cells blocked A23187-induced striatal cell death in a concentration-dependent manner. These results suggest that oxidative stress, opening of the mitochondrial permeability transition pore and activation of caspases are important steps in A23187-induced cell death. Copyright (C) 2000 Elsevier Science B.V.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Apoptosis
- Calcium
- Cell death
- Huntington's disease
- Mitochondria
- Striatal neuron
Publication and Content Type
- art (subject category)
- ref (subject category)
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