Search: onr:"swepub:oai:lup.lub.lu.se:3965a8a0-d884-4df6-8f8b-104c7b29b865" > The G protein-coupl...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 04846naa a2200433 4500 | |
001 | oai:lup.lub.lu.se:3965a8a0-d884-4df6-8f8b-104c7b29b865 | |
003 | SwePub | |
008 | 160401s2012 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/24314242 URI |
024 | 7 | a https://doi.org/10.1007/s11010-012-1301-32 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Holm, Andersu Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups4 aut0 (Swepub:lu)med-aeh |
245 | 1 0 | a The G protein-coupled oestrogen receptor 1 agonist G-1 disrupts endothelial cell microtubule structure in a receptor-independent manner. |
264 | c 2012-03-27 | |
264 | 1 | b Springer Science and Business Media LLC,c 2012 |
338 | a electronic2 rdacarrier | |
520 | a The G protein-coupled oestrogen receptor GPER1, also known as GPR30, has been implicated in oestrogen signalling, but the physiological importance of GPER1 is not fully understood. The GPER1 agonist G-1 has become an important tool to assess GPER1-mediated cellular effects. Here, we report that this substance, besides acting via GPER1, affects the microtubule network in endothelial cells. Treatment with G-1 (3 μM) for 24 h reduced DNA synthesis by about 60 % in mouse microvascular endothelial bEnd.3 cells. Treatment with 3 μM G-1 prevented outgrowth of primary endothelial cells from mouse aortic explants embedded in Matrigel. Treatment with G-1 (0.3-3 μM) for 24 h disrupted bEnd.3 cell and HUVEC microtubule structure in a concentration-dependent manner as assessed by laser-scanning confocal immunofluorescence microscopy. G-1-induced (3 μM) disruption of microtubule was observed also after acute (3 and 6 h) treatment and in the presence of the protein synthesis inhibitor cycloheximide. Disruption of microtubules by 3 μM G-1 was observed in aortic smooth muscle cells obtained from both GPER1 knockout and wild-type mice, suggesting that G-1 influences microtubules through a mechanism independent of GPER1. G-1 dose dependently (10-50 μM) stimulated microtubule assembly in vitro. On the other hand, microtubules appeared normal in the presence of 10-50 μM G-1 as determined by electron microscopy. We suggest that G-1-promoted endothelial cell anti-proliferation is due in part to alteration of microtubule organization through a mechanism independent of GPER1. This G-1-promoted mechanism may be used to block unwanted endothelial cell proliferation and angiogenesis such as that observed in, e.g. cancer. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Kardiologi0 (SwePub)302062 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cardiac and Cardiovascular Systems0 (SwePub)302062 hsv//eng |
700 | 1 | a Grände, Per-Olofu Lund University,Lunds universitet,Anestesiologi och intensivvård,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Anesthesiology and Intensive Care,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)mphy-pog |
700 | 1 | a Ludueña, Richard F4 aut |
700 | 1 | a Olde, Björnu Lund University,Lunds universitet,Kardiologi,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Cardiology,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)mphy-bol |
700 | 1 | a Prasad, Veena4 aut |
700 | 1 | a Leeb-Lundberg, Fredriku Lund University,Lunds universitet,Drug Target Discovery,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)mphy-fle |
700 | 1 | a Nilsson, Bengt-Olofu Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-bon |
710 | 2 | a Kärlfysiologib Forskargrupper vid Lunds universitet4 org |
773 | 0 | t Molecular and Cellular Biochemistryd : Springer Science and Business Media LLCg 366:1-2, s. 239-249q 366:1-2<239-249x 0300-8177x 1573-4919 |
856 | 4 | u https://portal.research.lu.se/files/4248905/2861104.pdfx primaryx freey FULLTEXT |
856 | 4 | u http://www.ncbi.nlm.nih.gov/pubmed/22451019?dopt=Abstracty FULLTEXT |
856 | 4 | u http://dx.doi.org/10.1007/s11010-012-1301-3y FULLTEXT |
856 | 4 | u https://lup.lub.lu.se/search/files/4248905/2861104.pdf |
856 | 4 8 | u https://lup.lub.lu.se/record/2431424 |
856 | 4 8 | u https://doi.org/10.1007/s11010-012-1301-3 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
Copy and save the link in order to return to this view