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  • Ramírez-Torres, AlbertoNational Autonomous University of Mexico (author)

Quantitative Proteomic Analysis of Cervical Cancer Tissues Identifies Proteins Associated With Cancer Progression

  • Article/chapterEnglish2022

Publisher, publication year, extent ...

  • 2022-02-18
  • Anticancer Research USA Inc.2022
  • 18 s.

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:39a0859e-2c00-463e-a78d-4590e051bd74
  • https://lup.lub.lu.se/record/39a0859e-2c00-463e-a78d-4590e051bd74URI
  • https://doi.org/10.21873/cgp.20317DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Background/Aim: To date, several proteomics studies in cervical cancer (CC) have focused mainly on squamous cervical cancer (SCC). Our study aimed to discover and clarify differences in SCC and CAD that may provide valuable information for the identification of proteins involved in tumor progression, in CC as a whole, or specific for SCC or CAD. Materials and Methods: Total protein extracts from 15 individual samples corresponding to 5 different CC tissue types were compared with a non-cancerous control group using bidimensional liquid chromatography-mass spectrometry (2D LC-MS/MS), isobaric tags for relative and absolute quantitation (ITRAQ), principal component analysis (PCA) and gene set enrichment analysis (GSEA). Results: A total of 622 statistically significant different proteins were detected. Exocytosis-related proteins were the most over-represented, accounting for 25% of the identified and quantified proteins. Based on the experimental results, reticulocalbin 3 (RCN3) and Ras-related protein Rab-14 (RAB14) were chosen for further downstream in vitro and vivo analyses. RCN3 was overexpressed in all CC tissues compared to the control and RAB14 was overexpressed in squamous cervical cancer (SCC) compared to invasive cervical adenocarcinoma (CAD). In the tumor xenograft experiment, RAB14 protein expression was positively correlated with increased tumor size. In addition, RCN3-expressing HeLa cells induced a discrete size increment compared to control, at day 47 after inoculation. Conclusion: RAB14 and RCN3 are suggested as potential biomarkers and therapeutic targets in the treatment of CC.

Subject headings and genre

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  • Gil, JeovanisLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Clinical Chemistry, Malmö,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,National Autonomous University of Mexico(Swepub:lu)je2062gi (author)
  • Contreras, SandraNational Autonomous University of Mexico (author)
  • Ramírez, GracielaSchulthess Clinic (author)
  • Valencia-González, Heriberto A.Schulthess Clinic (author)
  • Salazar-Bustamante, EmmanuelNational Autonomous University of Mexico (author)
  • Gómezcaudillo, LeopoldoNational Autonomous University of Mexico (author)
  • García-Carranca, AlejandroSchulthess Clinic (author)
  • Encarnación-Guevara, SergioNational Autonomous University of Mexico (author)
  • National Autonomous University of MexicoKlinisk kemi, Malmö (creator_code:org_t)

Related titles

  • In:Cancer Genomics and Proteomics: Anticancer Research USA Inc.19:2, s. 241-2581109-65351790-6245

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