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ACouWash : A standalone instrument for the washing, separation and enrichment of cells.
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- Mallinson, Jay (author)
- AcouSort AB
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- Linander, Oskar (author)
- AcouSort AB
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- Magnusson, Cecilia (author)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Acoustofluidics group,Clinical Chemistry, Malmö,Lund University Research Groups,AcouSort AB
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- (creator_code:org_t)
- 2018
- 2018
- English 3 s.
- In: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - 9781510897571 ; 1, s. 279-281
- Conference paper (peer-reviewed)
Abstract
Subject headings
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- We developed the AcouWash (Figures 1 and 2), a benchtop instrument for flow-through cell and particle separation by acoustophoresis. Building on previous research [1-4] AcouWash can re-suspend cells in new medium, separate cells based on size or acoustic properties, or enrich cells, Figure 3. We present our initial evaluation of AcouWash regarding size-based separation of particles, cell washing, cell viability, and time savings compared to centrifugation. We repeatedly recovered 90-100% of target cells and particles, washed away 99.9% of a small fluorescent tracer molecule, viability of cells was unchanged, and reduced the time for standard cell labelling by 50%.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Annan medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Other Medical Biotechnology (hsv//eng)
Keyword
- Acoustofluidics
- Acoustophoresis
- Blood cells
- Enrichment
- Separation
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- kon (subject category)
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