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- Ekström, SimonLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH (author)
On-chip microextraction for proteomic sample preparation of in-gel digests
- Article/chapterEnglish2002
Publisher, publication year, extent ...
- 2002
- 9 s.
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:3d964ccc-c2d8-4673-9775-29ee38ea3ac4
- https://lup.lub.lu.se/record/338799URI
- https://doi.org/10.1002/1615-9861(200204)2:4<413::AID-PROT413>3.0.CO;2-1DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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- Despite the high sensitivity and relatively high tolerance for contaminants of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) there is often a need to purify and concentrate the sample solution, especially after in-gel digestion of proteins separated by two-dimensional gel electrophoresis (2-DE). A silicon microextraction chip (SMEC) for sample clean-up and trace enrichment of peptides was manufactured and investigated. The microchip structure was used to trap reversed-phase chromatography media (POROS R2 beads) that facilitates sample purification/enrichment of contaminated and dilute samples prior to the MALDI-TOF MS analysis. The validity of the SMEC sample preparation technique was successfully investigated by performing analysis on a 10 nM peptide mixture containing 2 m urea in 0.1 m phosphate-buffered saline with MALDI-TOF MS. It is demonstrated that the microchip sample clean-up and enrichment of peptides can facilitate identification of proteins from 2-DE separations. The microchip structure was also used to trap beads immobilized with trypsin, thereby effectively becoming a microreactor for enzymatic digestion of proteins. This microreactor was used to generate a peptide map from a 100 nM bovine serum albumin sample.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine hsv//eng
- Electrophoresis, Gel, Two-Dimensional
- Enzymes, Immobilized
- Peptides
- Protein Array Analysis
- Proteome
- Reproducibility of Results
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Evaluation Studies
- Journal Article
- Research Support, Non-U.S. Gov't
Added entries (persons, corporate bodies, meetings, titles ...)
- Malmström, JohanLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine Proteomics,Forskargrupper vid Lunds universitet,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups(Swepub:lu)medk-jma (author)
- Wallman, LarsLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Elektroteknik (CI),Utbildningsprogram, LTH,Neuronano Research Center (NRC),Forskargrupper vid Lunds universitet,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Electrical Engineering (M.Sc.Eng.),Educational programmes, LTH,Faculty of Engineering, LTH,Lund University Research Groups(Swepub:lu)elma-lwa (author)
- Löfgren, Mikael (author)
- Nilsson, JohanLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-jni (author)
- Laurell, ThomasLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-tla (author)
- Marko-Varga, GyörgyLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)akem-gmv (author)
- Avdelningen för Biomedicinsk teknikInstitutionen för biomedicinsk teknik (creator_code:org_t)
Related titles
- In:Proteomics2:4, s. 413-4211615-9861
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