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Identification of c...
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Shi, JinruiSamuel Roberts Noble Foundation
(author)
Identification of cDNA clones encoding vasolin-containing protein and other plant plasma membrane-associated proteins by a general immuno-screening strategy.
- Article/chapterEnglish1995
Publisher, publication year, extent ...
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1995-05-09
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Proceedings of the National Academy of Sciences,1995
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5 s.
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LIBRIS-ID:oai:lup.lub.lu.se:40565651-5a6f-4149-b7d2-d023bfab77dc
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https://lup.lub.lu.se/record/40565651-5a6f-4149-b7d2-d023bfab77dcURI
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https://doi.org/10.1073/pnas.92.10.4457DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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An approach was developed for the isolation and characterization of soybean plasma membrane-associated proteins by immunoscreening of a cDNA expression library. An antiserum was raised against purified plasma membrane vesicles. In a differential screening of approximately 500,000 plaque-forming units with the anti-(plasma membrane) serum and DNA probes derived from highly abundant clones isolated in a preliminary screening, 261 clones were selected from approximately 1,200 antiserum-positive plaques. These clones were classified into 40 groups by hybridization analysis and 5'- and 3'-terminal sequencing. By searching nucleic acid and protein sequence data bases, 11 groups of cDNAs were identified, among which valosin-containing protein (VCP), clathrin heavy chain, phospholipase C, and S-adenosylmethionine:delta 24-sterol-C-methyltransferase have not to date been cloned from plants. The remaining 29 groups did not match any current data base entries and may, therefore, represent additional or yet uncharacterized genes. A full-length cDNA encoding the soybean VCP was sequenced. The high level of amino acid identity with vertebrate VCP and yeast CDC48 protein indicates that the soybean protein is a plant homolog of vertebrate VCP and yeast CDC48 protein.
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Dixon, Richard A.Samuel Roberts Noble Foundation
(author)
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Gonzales, Robert A.Samuel Roberts Noble Foundation
(author)
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Kjellbom, PerLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)pbio-pkj
(author)
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Bhattacharyya, Madan K.Samuel Roberts Noble Foundation
(author)
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Samuel Roberts Noble FoundationBiokemi och Strukturbiologi
(creator_code:org_t)
Related titles
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In:Proceedings of the National Academy of Sciences: Proceedings of the National Academy of Sciences92, s. 4457-44611091-64900027-8424
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