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Reaction medium eng...
Reaction medium engineering in enzymatic peptide fragment condensation : Synthesis of eledoisin and LH-RH
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- Björup, Peter (author)
- CSIC Centro de Investigación y Desarrollo Pascual Vila (CID)
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- Torres, Josep Lluís (author)
- CSIC Centro de Investigación y Desarrollo Pascual Vila (CID)
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- Adlercreutz, Patrick (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Clapés, Pere (author)
- CSIC Centro de Investigación y Desarrollo Pascual Vila (CID)
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(creator_code:org_t)
- 1998
- 1998
- English 11 s.
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In: Bioorganic and Medicinal Chemistry. - 0968-0896. ; 6:7, s. 891-901
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Abstract
Subject headings
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- The influence of different reaction systems on α-chymotrypsin-catalyzed synthesis of eledoisin and LH-RH peptides from (7+4) and (5+5) fragments was investigated. The peptide yield was determined in the following systems: buffered aqueous media, frozen solutions, organic media, and cosolvent mixtures. The experimental set up was tailored to allow the screening of an array of conditions with minimum consumption of peptide fragments (2.1 and 2.5mM). The best yields (22% yield for eledoisin and 68% yield for LH-RH) were obtained in buffered aqueous solutions. It was found that the choice of buffer had a strong influence on the peptide yield; boric-borate and ammonium acetate buffers at pH 9, gave the best results. In buffered aqueous systems, both syntheses were scaled up by using a 10-fold increase in fragment concentration (21 and 25mM). Under these conditions the yields rose to 57% and 80% of eledoisin and LH-RH, respectively. Moreover, during the synthesis of eledoisin and in the presence of boric-borate buffer pH 9, the peptide precipitated from the reaction medium preventing a secondary hydrolysis and facilitating the in situ product purification. Copyright (C) 1998 Elsevier Science Ltd.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Keyword
- α-chymotrypsin
- Aqueous medium
- Biologically active peptides
- Cam esters
- Eledoisin
- Inverse substrate
- Kinetically controlled fragment condensation
- Luteinising hormone releasing hormone (LH-RH)
- Trypsin
Publication and Content Type
- art (subject category)
- ref (subject category)
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