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Amyloid beta-Protei...
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Hellstrand, ErikLund University,Lunds universitet,Biofysikalisk kemi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biophysical Chemistry,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
(author)
Amyloid beta-Protein Aggregation Produces Highly Reproducible Kinetic Data and Occurs by a Two-Phase Process
- Article/chapterEnglish2010
Publisher, publication year, extent ...
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2009-10-09
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American Chemical Society (ACS),2010
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LIBRIS-ID:oai:lup.lub.lu.se:44ac5a93-b3e4-46ac-b657-1da13135d03f
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https://lup.lub.lu.se/record/1617649URI
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https://doi.org/10.1021/cn900015vDOI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Protein aggregation can lead to major disturbances of cellular processes and is associated with several diseases. We report kinetic and equilibrium data by ThT fluorescence and enzyme-linked immunosorbent assay of sufficient quality and reproducibility to form a basis for mechanistic understanding of amyloid beta-peptide (A beta) fibril formation. Starting from monomeric peptide in a pure buffer system without cosolvents, we find that the kinetics of A beta aggregation vary strongly with peptide concentration in a highly predictable manner. The free A beta concentration in equilibrium with fibrils was found to vary with total peptide concentration in a manner expected for a two-phase system. The free versus total A beta concentration was linear up to ca. 0.2,mu M, after which free A beta decreased with total A beta toward an asymptotic value. Our results imply that A beta fibril formation arises from a sequence of events in a highly predictable manner.
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Boland, Barry
(author)
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Walsh, Dominic M.
(author)
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Linse, SaraLund University,Lunds universitet,Biofysikalisk kemi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biophysical Chemistry,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)fkm2-sli
(author)
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Biofysikalisk kemiCentrum för Molekylär Proteinvetenskap
(creator_code:org_t)
Related titles
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In:ACS Chemical Neuroscience: American Chemical Society (ACS)1:1, s. 13-181948-7193
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