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Nano-encapsulated E...
Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ
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- Lee, Sarah C. (author)
- University of Birmingham
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- Collins, Richard (author)
- University of Manchester
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- Lin, Yu-pin (author)
- University of Birmingham
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- Jamshad, Mohammed (author)
- University of Birmingham
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- Broughton, Claire (author)
- University of Warwick
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- Harris, Sarah A. (author)
- University of Leeds
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- Hanson, Benjamin S. (author)
- University of Leeds
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- Tognoloni, Cecilia (author)
- University of Bath
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- Parslow, Rosemary A. (author)
- University of Birmingham
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- Terry, Ann E. (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory
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- Rodger, Alison (author)
- Macquarie University, Sydney
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- Smith, Corinne J. (author)
- University of Warwick
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- Edler, Karen J. (author)
- University of Bath
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- Ford, Robert (author)
- University of Manchester
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- Roper, David I. (author)
- University of Warwick
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- Dafforn, Timothy R. (author)
- University of Birmingham
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(creator_code:org_t)
- 2019-12-10
- 2019
- English 16 s.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9:1
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https://www.nature.c...
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Abstract
Subject headings
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- The E. coli membrane protein ZipA, binds to the tubulin homologue FtsZ, in the early stage of cell division. We isolated ZipA in a Styrene Maleic Acid lipid particle (SMALP) preserving its position and integrity with native E. coli membrane lipids. Direct binding of ZipA to FtsZ is demonstrated, including FtsZ fibre bundles decorated with ZipA. Using Cryo-Electron Microscopy, small-angle X-ray and neutron scattering, we determine the encapsulated-ZipA structure in isolation, and in complex with FtsZ to a resolution of 1.6 nm. Three regions can be identified from the structure which correspond to, SMALP encapsulated membrane and ZipA transmembrane helix, a separate short compact tether, and ZipA globular head which binds FtsZ. The complex extends 12 nm from the membrane in a compact structure, supported by mesoscale modelling techniques, measuring the movement and stiffness of the regions within ZipA provides molecular scale analysis and visualisation of the early divisome.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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- ref (subject category)
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- By the author/editor
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Lee, Sarah C.
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Collins, Richard
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Lin, Yu-pin
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Jamshad, Mohamme ...
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Broughton, Clair ...
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Harris, Sarah A.
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show more...
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Hanson, Benjamin ...
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Tognoloni, Cecil ...
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Parslow, Rosemar ...
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Terry, Ann E.
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Rodger, Alison
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Smith, Corinne J ...
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Edler, Karen J.
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Ford, Robert
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Roper, David I.
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Dafforn, Timothy ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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Scientific Repor ...
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Lund University