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  • Akbari, ElhamLund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Department of Physics,Departments at LTH,Faculty of Engineering, LTH (author)

SEPARATION OF SINGLETS AND CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT AND FILTER SONICATION

  • Article/chapterEnglish2022

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  • 2022
  • 2 s.

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  • LIBRIS-ID:oai:lup.lub.lu.se:484f1a63-6814-42d3-b614-de3ca9b0a240
  • https://lup.lub.lu.se/record/484f1a63-6814-42d3-b614-de3ca9b0a240URI

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  • Language:English
  • Summary in:English

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  • Subject category:kon swepub-publicationtype
  • Subject category:ref swepub-contenttype

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  • Differences in morphologies of bacteria and bacteria clusters are thought to contribute to their virulence and colonization. However, the conventional standard cell biological methods cannot separate bacteria and bacteria clusters based on their morphologies and sizes, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of singlets and clusters, of group A streptococci, based on their size and morphology, using Deterministic Lateral Displacement and filter-sonication. In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.

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  • Beech, Jason P.Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Department of Physics,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ftf-job (author)
  • Wrighton, SebastianLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Kvantitativ immunbiologi,Forskargrupper vid Lunds universitet,epIgG,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Quantitative immunobiology,Lund University Research Groups(Swepub:lu)se7062wr (author)
  • Nordenfelt, PontusLund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Other operations, LTH,Faculty of Engineering, LTH,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)med-pnl (author)
  • Tegenfeldt, Jonas O.Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Department of Physics,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ftf-jte (author)
  • NanoLund: Centre for NanoscienceAnnan verksamhet, LTH (creator_code:org_t)

Related titles

  • In:MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, s. 306-3079781733419048

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