Search: onr:"swepub:oai:lup.lub.lu.se:485c5b61-e15d-40b2-a5a7-ceee454c3f87" >
A serological type ...
A serological type II collagen neoepitope biomarker reflects cartilage breakdown in patients with osteoarthritis
-
- Groen, Solveig Skovlund (author)
- Nordic Bioscience AS,University of Copenhagen
-
- Sinkeviciute, Dovile (author)
- Lund University,Lunds universitet,Reumatologi och molekylär skelettbiologi,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Rheumatology,Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Molecular Skeletal Biology,Lund University Research Groups,Nordic Bioscience AS
-
- Bay-Jensen, Anne Christine (author)
- Nordic Bioscience AS
-
show more...
-
- Thudium, Christian S. (author)
- Nordic Bioscience AS
-
- Karsdal, Morten A. (author)
- Nordic Bioscience AS
-
- Thomsen, Simon Francis (author)
- Bispebjerg Hospital
-
- Lindemann, Sven (author)
- Merck KGaA
-
- Werkmann, Daniela (author)
- Merck KGaA
-
- Blair, Joseph (author)
- Nordic Bioscience AS
-
- Staunstrup, Line Mærsk (author)
- Nordic Bioscience AS
-
- Önnerfjord, Patrik (author)
- Lund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups
-
- Arendt-Nielsen, Lars (author)
- Aalborg University
-
- Nielsen, Signe Holm (author)
- Technical University of Denmark,Nordic Bioscience AS
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English.
-
In: Osteoarthritis and Cartilage Open. - : Elsevier BV. - 2665-9131. ; 3:4
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
https://doi.org/10.1...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Objectives: There is an unmet medical need for biomarkers in OA which can be applied in clinical drug development trials. The present study describes the development of a specific and robust assay measuring type II collagen degradation (T2CM) and discusses its potential as a noninvasive translational biomarker. Methods: A type II collagen specific neoepitope (T2CM) was identified by mass spectrometry and monoclonal antibodies were raised towards the epitope, employed in a chemiluminescence immunoassay. T2CM was assessed in bovine cartilage explants with or without MMP-13 inhibitor, and explant supernatants were analyzed by Western blot. T2CM was measured in plasma samples from one study (n = 48 patients) where OA patients were referred to total knee replacement (TKR). Additionally, T2CM was quantified in serum from OA patients receiving salmon calcitonin treatment (sCT) (n = 50) compared to placebo (n = 57). Results: The T2CM assay was technically robust (13/4 % inter/intra-variation) and specific for the type II collagen fragment cleaved by MMP-1 and -13. The MMP-13 inhibitor reduced the T2CM release from bovine cartilage explants receiving catabolic treatment. These results were confirmed by Western blot. In human end-stage OA patients (scheduled for TKR), the T2CM levels were elevated compared to moderate OA (p<0.004). The OA patients receiving sCT had lower levels of T2CM compared to placebo group after 1, 6, and 24 months of treatment (p = 0.0285, p = 0.0484, p = 0.0035). Conclusions: To our knowledge, T2CM is the first technically robust serological biomarker assay which has shown biological relevance in ex vivo models and OA cohorts. This suggests that T2CM may have potential as a translational biomarker for cartilage degradation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Biomarker
- Cartilage
- Extracellular matrix
- T2CM
- Type II collagen
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Groen, Solveig S ...
-
Sinkeviciute, Do ...
-
Bay-Jensen, Anne ...
-
Thudium, Christi ...
-
Karsdal, Morten ...
-
Thomsen, Simon F ...
-
show more...
-
Lindemann, Sven
-
Werkmann, Daniel ...
-
Blair, Joseph
-
Staunstrup, Line ...
-
Önnerfjord, Patr ...
-
Arendt-Nielsen, ...
-
Nielsen, Signe H ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Osteoarthritis a ...
- By the university
-
Lund University