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- Jacobsen, S. C. (author)
Effects of short-term high-fat overfeeding on genome-wide DNA methylation in the skeletal muscle of healthy young men
- Article/chapterEnglish2012
Publisher, publication year, extent ...
- 2012-09-08
- Springer Science and Business Media LLC,2012
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:4b72588b-844d-45b3-9f48-7da15d629c5d
- https://lup.lub.lu.se/record/3379424URI
- https://doi.org/10.1007/s00125-012-2717-8DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- Energy-dense diets that are high in fat are associated with a risk of metabolic diseases. The underlying molecular mechanisms could involve epigenetics, as recent data show altered DNA methylation of putative type 2 diabetes candidate genes in response to high-fat diets. We examined the effect of a short-term high-fat overfeeding (HFO) diet on genome-wide DNA methylation patterns in human skeletal muscle. Skeletal muscle biopsies were obtained from 21 healthy young men after ingestion of a short-term HFO diet and a control diet, in a randomised crossover setting. DNA methylation was measured in 27,578 CpG sites/14,475 genes using Illumina's Infinium Bead Array. Candidate gene expression was determined by quantitative real-time PCR. HFO introduced widespread DNA methylation changes affecting 6,508 genes (45%), with a maximum methylation change of 13.0 percentage points. The HFO-induced methylation changes were only partly and non-significantly reversed after 6-8 weeks. Alterations in DNA methylation levels primarily affected genes involved in inflammation, the reproductive system and cancer. Few gene expression changes were observed and these had poor correlation to DNA methylation. The genome-wide DNA methylation changes induced by the short-term HFO diet could have implications for our understanding of transient epigenetic regulation in humans and its contribution to the development of metabolic diseases. The slow reversibility suggests a methylation build-up with HFO, which over time may influence gene expression levels.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
- DNA methylation
- Epigenetic
- Gene expression
- Genome-wide
- High-fat
- overfeeding
- Human
- Illumina Infinium 27k Bead Array
- Reversibility
- Skeletal muscle
- Type 2 diabetes
Added entries (persons, corporate bodies, meetings, titles ...)
- Brons, C. (author)
- Bork-Jensen, J. (author)
- Ribel-Madsen, R. (author)
- Yang, BeatriceLund University,Lunds universitet,Genomik, diabetes och endokrinologi,Forskargrupper vid Lunds universitet,Genomics, Diabetes and Endocrinology,Lund University Research Groups(Swepub:lu)be3734ya (author)
- Lara, E. (author)
- Hall, ElinLund University,Lunds universitet,Genomik, diabetes och endokrinologi,Forskargrupper vid Lunds universitet,Genomics, Diabetes and Endocrinology,Lund University Research Groups(Swepub:lu)med-ehl (author)
- Calvanese, V. (author)
- Nilsson, E. (author)
- Jorgensen, S. W. (author)
- Mandrup, S. (author)
- Ling, CharlotteLund University,Lunds universitet,Genomik, diabetes och endokrinologi,Forskargrupper vid Lunds universitet,Genomics, Diabetes and Endocrinology,Lund University Research Groups(Swepub:lu)endo-cl0 (author)
- Fernandez, A. F. (author)
- Fraga, M. F. (author)
- Poulsen, P. (author)
- Vaag, A. (author)
- Genomik, diabetes och endokrinologiForskargrupper vid Lunds universitet (creator_code:org_t)
Related titles
- In:Diabetologia: Springer Science and Business Media LLC55:12, s. 3341-33491432-04280012-186X
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- Jacobsen, S. C.
- Brons, C.
- Bork-Jensen, J.
- Ribel-Madsen, R.
- Yang, Beatrice
- Lara, E.
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- Hall, Elin
- Calvanese, V.
- Nilsson, E.
- Jorgensen, S. W.
- Mandrup, S.
- Ling, Charlotte
- Fernandez, A. F.
- Fraga, M. F.
- Poulsen, P.
- Vaag, A.
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- Diabetologia
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