Search: onr:"swepub:oai:lup.lub.lu.se:4e16a82a-44d6-4160-b048-9cc8a9ce1328" >
Universal intracell...
Universal intracellular biomolecule delivery with precise dosage control
-
- Cao, Y. (author)
- Stanford University
-
- Chen, H. (author)
- Stanford University
-
- Qiu, R. (author)
- Stanford University
-
show more...
-
- Hanna, M. (author)
- Stanford University
-
- Ma, E. (author)
- University of California, Berkeley
-
- Hjort, M. (author)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Synkrotronljusfysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Synchrotron Radiation Research,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
-
- Zhang, A. (author)
- Stanford University
-
- Lewis, R. S. (author)
- Stanford University
-
- Wu, J. C. (author)
- Stanford University
-
- Melosh, N. A. (author)
- Stanford University
-
show less...
-
(creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2018
- 2018
- English.
-
In: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 4:10
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
https://advances.sci...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Intracellular delivery of mRNA, DNA, and other large macromolecules into cells plays an essential role in an array of biological research and clinical therapies. However, currentmethods yield a wide variation in the amount of material delivered, as well as limitations on the cell types and cargoes possible. Here, we demonstrate quantitatively controlled delivery into a range of primary cells and cell lines with a tight dosage distribution using a nanostrawelectroporation system (NES). In NES, cells are cultured onto track-etched membranes with protruding nanostraws that connect to the fluidic environment beneath the membrane. The tight cell-nanostraw interface focuses applied electric fields to the cell membrane, enabling low-voltage and nondamaging local poration of the cell membrane. Concurrently, the field electrophoretically injects biomolecular cargoes through the nanostraws and into the cell at the same location. We show that the amount of material delivered is precisely controlled by the applied voltage, delivery duration, and reagent concentration. NES is highly effective even for primary cell types or different cell densities, is largely cargo agnostic, and can simultaneously deliver specific ratios of different molecules. Using a simple cell culture well format, the NES delivers into >100,000 cells within 20 s with >95% cell viability, enabling facile, dosage-controlled intracellular delivery for a wide variety of biological applications.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Cao, Y.
-
Chen, H.
-
Qiu, R.
-
Hanna, M.
-
Ma, E.
-
Hjort, M.
-
show more...
-
Zhang, A.
-
Lewis, R. S.
-
Wu, J. C.
-
Melosh, N. A.
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Science Advances
- By the university
-
Lund University