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Guidelines for DC p...
Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues
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- Probst, Hans Christian (author)
- Universitätsmedizin Mainz
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- Stoitzner, Patrizia (author)
- Medical University of Innsbruck
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- Amon, Lukas (author)
- University Hospital Erlangen
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- Backer, Ronald A. (author)
- Universitätsmedizin Mainz
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- Brand, Anna (author)
- Universitätsmedizin Mainz
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- Chen, Jianzhou (author)
- Institut Gustave Roussy
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- Clausen, Björn E. (author)
- Universitätsmedizin Mainz
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- Dieckmann, Sophie (author)
- Medical University of Innsbruck
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- Dudziak, Diana (author)
- Friedrich-Alexander University Erlangen-Nürnberg,University Hospital Erlangen
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- Heger, Lukas (author)
- University Hospital Erlangen
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- Hodapp, Katrin (author)
- Universitätsmedizin Mainz
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- Hornsteiner, Florian (author)
- Medical University of Innsbruck
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- Hovav, Avi Hai (author)
- Hebrew University of Jerusalem
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- Jacobi, Lukas (author)
- University Hospital Erlangen
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- Ji, Xingqi (author)
- Ludwig-Maximilian University of Munich,University Hospital Munich
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- Kamenjarin, Nadine (author)
- Universitätsmedizin Mainz
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- Lahl, Katharina (author)
- Lund University,Lunds universitet,Virusrekognition,Forskargrupper vid Lunds universitet,Virus Recognition,Lund University Research Groups,Technical University of Denmark
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- Lahmar, Imran (author)
- Institut Gustave Roussy
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- Lakus, Jelena (author)
- Universitätsmedizin Mainz
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- Lehmann, Christian H.K. (author)
- University Hospital Erlangen
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- Ortner, Daniela (author)
- Medical University of Innsbruck
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- Picard, Marion (author)
- Institut Gustave Roussy
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- Roberti, Maria Paula (author)
- German Cancer Research Centre,Institut Gustave Roussy,University Hospital Heidelberg
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- Rossnagel, Lukas (author)
- University Hospital Erlangen
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- Saba, Yasmin (author)
- Hebrew University of Jerusalem
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- Schalla, Carmen (author)
- RWTH Aachen University
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- Schlitzer, Andreas (author)
- University of Bonn
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- Schraml, Barbara U. (author)
- Ludwig-Maximilian University of Munich,University Hospital Munich
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- Schütze, Kristian (author)
- Universitätsmedizin Mainz
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- Seichter, Anna (author)
- University Hospital Erlangen
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- Seré, Kristin (author)
- RWTH Aachen University
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- Seretis, Athanasios (author)
- University of Innsbruck
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- Sopper, Sieghart (author)
- Medical University of Innsbruck
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- Strandt, Helen (author)
- Medical University of Innsbruck
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- Sykora, Martina M. (author)
- Medical University of Innsbruck
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- Theobald, Hannah (author)
- University of Bonn
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- Tripp, Christoph H. (author)
- Medical University of Innsbruck
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- Zitvogel, Laurence (author)
- Institut Gustave Roussy
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(creator_code:org_t)
- 2022-12-13
- 2023
- English.
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In: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 53:11
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues. DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in-depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Dendritic cells
- Discrimination of dendritic cell subsets
- Mouse nonlymphoid tissue
- Multiplex flow cytometry analysis
- Tissue digestion protocols
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Probst, Hans Chr ...
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Stoitzner, Patri ...
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Amon, Lukas
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Backer, Ronald A ...
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Brand, Anna
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Chen, Jianzhou
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Clausen, Björn E ...
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Dieckmann, Sophi ...
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Dudziak, Diana
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Heger, Lukas
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Hodapp, Katrin
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Hornsteiner, Flo ...
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Hovav, Avi Hai
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Jacobi, Lukas
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Ji, Xingqi
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Kamenjarin, Nadi ...
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Lahl, Katharina
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Lahmar, Imran
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Lakus, Jelena
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Lehmann, Christi ...
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Ortner, Daniela
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Picard, Marion
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Roberti, Maria P ...
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Rossnagel, Lukas
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Saba, Yasmin
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Schalla, Carmen
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Schlitzer, Andre ...
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Schraml, Barbara ...
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Schütze, Kristia ...
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Seichter, Anna
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Seré, Kristin
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Seretis, Athanas ...
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Sopper, Sieghart
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Strandt, Helen
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Sykora, Martina ...
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Theobald, Hannah
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Tripp, Christoph ...
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Zitvogel, Lauren ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
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European Journal ...
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Lund University