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A Method to Study t...
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Weishaupt, HolgerLund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
(author)
A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip
- Article/chapterEnglish2010
Publisher, publication year, extent ...
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2010-05-15
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Springer Science and Business Media LLC,2010
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electronicrdacarrier
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LIBRIS-ID:oai:lup.lub.lu.se:52cd9a5d-fcfa-45d7-a89c-97e84c846671
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https://lup.lub.lu.se/record/1753441URI
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https://doi.org/10.1007/s12575-010-9031-yDOI
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Language:English
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Summary in:English
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Notes
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Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP-chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we describe the miniChIP methodology that can be used for increasing an understanding of the epigenetic mechanisms underlying hematopoietic stem and progenitor cell function. Application of this method will reveal the contribution of dynamic chromatin structure in regulating the function of other somatic stem cell populations, and how this process becomes perturbed in pathological conditions.
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Attema, JoanneLund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups(Swepub:lu)med-jae
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Institutionen för experimentell medicinsk vetenskapMedicinska fakulteten
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Related titles
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In:Biological Procedures Online: Springer Science and Business Media LLC12:1, s. 1-171480-9222
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