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IAP-Based Cell Sort...
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Lehnen, DanielaMiltenyi Biotec GmbH
(author)
IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells
- Article/chapterEnglish2017
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LIBRIS-ID:oai:lup.lub.lu.se:53856e88-a0a7-4f40-bd71-908794be95d6
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https://lup.lub.lu.se/record/53856e88-a0a7-4f40-bd71-908794be95d6URI
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https://doi.org/10.1016/j.stemcr.2017.08.016DOI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
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Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clinical translation of differentiation protocols requires standardization of production procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-associated protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP+ mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP+ cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP+ mesDA progenitors were more homogeneous in size and DA neuron density. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clinical cell replacement strategies. In this article, Knöbel and colleagues identified IAP as a cell surface marker for mesDA progenitor cells. Immunomagnetic sorting for IAP+ led to reproducible and homogeneous cell compositions. Intrastriatal transplantation of sorted cells at day 16 of differentiation in a PD rat model resulted in functional recovery, and grafts were more homogeneous in size and DA neuron density than unsorted cells.
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Barral, SerenaAix-Marseille University,University College London
(author)
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Cardoso, TiagoLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)med-tcd
(author)
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Grealish, ShaneLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)med-sge
(author)
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Heuer, AndreasLund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine(Swepub:lu)med-ah4
(author)
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Smiyakin, AndrejMiltenyi Biotec GmbH
(author)
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Kirkeby, AgneteLund University,Lunds universitet,Human neural utvecklingsbiologi,Forskargrupper vid Lunds universitet,Human Neural Developmental Biology,Lund University Research Groups(Swepub:lu)med-agk
(author)
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Kollet, JuttaMiltenyi Biotec GmbH
(author)
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Cremer, HaroldAix-Marseille University
(author)
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Parmar, MalinLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)mphy-mpa
(author)
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Bosio, AndreasMiltenyi Biotec GmbH
(author)
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Knöbel, SebastianMiltenyi Biotec GmbH
(author)
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Miltenyi Biotec GmbHAix-Marseille University
(creator_code:org_t)
Related titles
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In:Stem Cell Reports: Elsevier BV9:4, s. 1207-12202213-6711
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Lehnen, Daniela
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Barral, Serena
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Cardoso, Tiago
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Grealish, Shane
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Heuer, Andreas
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Smiyakin, Andrej
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Kirkeby, Agnete
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Kollet, Jutta
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Cremer, Harold
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Parmar, Malin
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Bosio, Andreas
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Knöbel, Sebastia ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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