Search: onr:"swepub:oai:lup.lub.lu.se:56103d3e-c756-440c-999b-2798a50028c7" >
Nucleotide-binding ...
Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor - Critical regulators of beta-defensins during helicobacter pylori infection
- Article/chapterEnglish2006
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:lup.lub.lu.se:56103d3e-c756-440c-999b-2798a50028c7
-
https://lup.lub.lu.se/record/411171URI
-
https://doi.org/10.1074/jbc.M510275200DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:art swepub-publicationtype
-
Subject category:ref swepub-contenttype
Notes
-
Host-pathogen interactions that allow Helicobacter pylori to survive and persist in the stomach of susceptible individuals remain unclear. Human beta-defensins ( hBDs), epithelial-derived antimicrobial peptides are critical components of host-defense at mucosal surfaces. The role of H. pylori-mediated NF-kappa B and epidermal growth factor receptor ( EGFR) activation on beta-defensin expression was investigated. Transient transfection studies utilizing beta-defensin promoter constructs were conducted in gastric cells with contribution of individual signaling events evaluated by the addition of specific inhibitors, small interference nucleotide-binding oligomerization domain 1( NOD1) RNA or plasmids encoding Vaccinia virus proteins that interrupt interleukin-1 and Toll-like receptor signaling. The role of individual MAPK pathways was further delineated in HEK-293 cells expressing conditional MAPK mutants. We found hBD2 expression exclusively dependent on the presence of the bacterial cag pathogenicity island, with NOD1 a critical host sensor. Impairment of murine beta-defensin 4( an orthologue of hBD2) expression in NOD1-deficient mice 7-days post-infection further confirmed the role of this cytoplasmic pattern-recognition receptor in eliciting host innate immunity. In contrast to hBD2, hBD3 expression was NOD1-independent but EGFR and ERK pathway-dependent. Importantly, Toll-like receptor signaling was not implicated in H. pylori-mediated hBD2 and hBD3 gene expression. The divergent signaling events governing hBD2 and hBD3 expression suggest temporal functional variation, such that hBD2 may contribute to antimicrobial barrier function during the inflammatory phase with hBD3 playing a greater role during the repair, wound healing phase of infection.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Argent, RH
(author)
-
Body-Malapel, M
(author)
-
Park, JH
(author)
-
Ewings, KE
(author)
-
Bowie, AG
(author)
-
Ong, SJ
(author)
-
Cook, SJ
(author)
-
Sørensen, Ole ELund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)medk-oso
(author)
-
Manzo, BA
(author)
-
Inohara, N
(author)
-
Klein, NJ
(author)
-
Nunez, G
(author)
-
Atherton, JC
(author)
-
Bajaj-Elliott, M
(author)
-
InfektionsmedicinSektion III
(creator_code:org_t)
Related titles
-
In:Journal of Biological Chemistry281:17, s. 11637-116481083-351X
Internet link
Find in a library
To the university's database
- By the author/editor
-
Boughan, PK
-
Argent, RH
-
Body-Malapel, M
-
Park, JH
-
Ewings, KE
-
Bowie, AG
-
show more...
-
Ong, SJ
-
Cook, SJ
-
Sørensen, Ole E
-
Manzo, BA
-
Inohara, N
-
Klein, NJ
-
Nunez, G
-
Atherton, JC
-
Bajaj-Elliott, M
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Infectious Medic ...
- Articles in the publication
-
Journal of Biolo ...
- By the university
-
Lund University